Browse > Article
http://dx.doi.org/10.5806/AST.2009.22.2.148

Enantiomer separation using a covalently immobilized chiral column derived from polysaccharide derivative by reversed phase liquid chromatography  

Huang, Hu (College of Pharmacy, Chosun University)
Jin, Jing Yu (College of Pharmacy, Chosun University)
Baek, Chae-Sun (College of Pharmacy, Chosun University)
Lee, Wonjae (College of Pharmacy, Chosun University)
Publication Information
Analytical Science and Technology / v.22, no.2, 2009 , pp. 148-151 More about this Journal
Abstract
Enantiomer separation of N-fluorenylmethoxycarbonyl (FMOC) $\alpha$-amino acid was performed on covalently immobilized chiral column (Chiralpak IB) based on polysaccharide derivative as a chiral selector by reversed phase liquid chromatography. The effect of the reversed mobile phase on the chromatographic parameters of the enantioselectivities, resolution factors and retention times using covalently immobilized Chiralpak IB was shown. Also the enantiomer separation of N-FMOC $\alpha$-amino acid in the reversed and normal phase was compared and the results obtained in the former mobile phase were generally lower than those in the latter mobile phase.
Keywords
enantiomer separation; chiral column; amino acid derivative; liquid chromatography;
Citations & Related Records
연도 인용수 순위
  • Reference
1 A. Ghanem, J. Sep. Sci., 30, 1019-1028(2007)   DOI   PUBMED   ScienceOn
2 T. Zhang, C. Kientzy, P. Franco, A. Ohnishi, Y. Kagamihara and H. J. Kurosawa, Chromatogr. A, 1075, 65-75 (2005)   DOI   PUBMED   ScienceOn
3 K.-A. Lee, S. Yeo, K. H. Kim, W. Lee and J. S. Kang, J. Pharm. Biomed. Anal., 46, 914-919(2008)   DOI   PUBMED   ScienceOn
4 J. Y. Jin, W. Lee, J. H. Park and J. J. Ryoo, J. Liq. Chrom. & Rel.Tech., 30, 1-9(2007)   DOI   ScienceOn
5 W. H. Pirkle and W. Lee, Bull. Kor. Chem. Soc., 19, 1277-1280(1998)
6 T. Zhang, D. Nguyen, P. Franco, T. Murakami, A. Ohnishi and H. Kurosawa, Anal. Chim. Acta, 557, 221-228 (2006)   DOI   ScienceOn
7 I. Ali and H. Y. Aboul-Enein, J. Sep. Sci., 29, 762-769(2006)   DOI   ScienceOn
8 Y. Okamoto and E. Yashima, Angew. Chem. Int. Ed., 37, 1020-1043(1998)   DOI   ScienceOn
9 T. Zhang, D. Nguyen, P. Franco, Y. Isobe, T. Michishita and T. Murakami, J. Pharma. Biomed. Anal., 46, 882-891(2008)   DOI   ScienceOn
10 J. Y. Jin, K.-A. Lee, J. S. Kang, Y. K. Kang, C.-S. Baek and W. Lee, Arch. Pharm. Res. 30, 654-664(2007)
11 E. Yashima, J. Chromatogr. A, 906, 105-125(2001)   DOI   PUBMED   ScienceOn