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http://dx.doi.org/10.5806/AST.2008.21.4.245

Structural determination of triterpenic acids in Prunellae Spica by fast atom bombardment tandem mass spectrometry  

Ahn, Young Min (College of Pharmacy, Kyung Hee University)
Lee, Kang Ro (College of Pharmacy, Sungkyunkwan University)
Hong, Jongki (College of Pharmacy, Kyung Hee University)
Publication Information
Analytical Science and Technology / v.21, no.4, 2008 , pp. 245-258 More about this Journal
Abstract
Five triterpenic acids as marker compounds were extracted and isolated from Prunellae Spica by column chromatography and reversed-phase high-performance liquid chromatography (HPLC), and their purity was determinated by HPLC (purity ${\geq}90%$). Molecular weight and elemental compositions of the five marker compounds were determined by fast atom bombardment high-resolution mass spectrometry (FAB-HRMS). The structural determination of the five marker compounds was carried out fast atom bombardment collision-induced dissociation tandem mass spectrometry (FAB-CID-MS/MS). The collision-induced dissociation (CID) of protonated molecules $[M+H]^+$ and deprotonated molecules $[M-H]^-$ produced diverse product ions due mainly to retro Diels-Alder reaction (RDA), dehydration and decarboxylation. Moreover, the CID-MS/MS spectra of the $[M-H]^-$ ions were observed charge-remote fragmentation (CRF) patterns. On the basis of interpretation of CID-MS/MS spectra, structural elucidation of triterpenic acids isolated from Prunellae Spica was clearly performed.
Keywords
Prunellae Spica; triterpenic acid; structural analysis; FAB-CID-MS/MS; dissociation patterns;
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