Browse > Article
http://dx.doi.org/10.5806/AST.2008.21.2.102

Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP-HPLC  

Tian, Minglei (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University)
Yan, Hongyuan (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University)
Row, Kyung Ho (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University)
Publication Information
Analytical Science and Technology / v.21, no.2, 2008 , pp. 102-108 More about this Journal
Abstract
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0-8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
Keywords
liquiritin; glycyrrhizic acid; glabridin; licorice; RP-HPLC;
Citations & Related Records
연도 인용수 순위
  • Reference
1 T. Bo, K. A. Li and H. Liu, Anal. Chimica Acta, 458, 345-354(2002)   DOI   ScienceOn
2 Y. W. Kim, S. H. Ki , J. R. Lee, S. J. Lee, C. W. Kim, S. C. Kim and S. G. Kim, Chemico-Bio. Interactions, 161, 125-138(2006).   DOI   ScienceOn
3 T. Tanahashi, T. Mune, H. Morita, H. Tanahashi, Y. Isomura, T. Suwa, H. Daido, C. E. Gomez-Sancehz and K. Yasuda, J. Steroid Biochem. Molecular Biology, 80, 441-447(2002)   DOI   ScienceOn
4 Y. Lee, C. Huang, and K. Wen, T. Suen, J. Chromatogr. A, 692, 137-145(1995)   DOI   ScienceOn
5 Y. Fujisawa, M. Sakamoto, M. Matsushita, T. Fujita and K. Nishioka, Microbiology and Immunology, 44, 799-804(2000)   DOI   PUBMED
6 B. Fu, J. Liu, H. Li, L. Li, F. S. C. Lee and X. Wang, J. Chromatogr. A, 1089, 18-24(2005)   DOI   ScienceOn
7 C. Sun, Y. Xie, Q. Tian and H. Liu, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 305(1-3), 42-47(2007)   DOI   ScienceOn
8 F. Aoki, K. Nakagawa, A. Tanaka, K. Matsuzaki, N. Arai and T. Mae, J. Chromatogr. B, 828, 70-74(2005)   DOI   ScienceOn
9 J. Cong and B. Lin, J. Chromatogr. A, 1145, 190-194 (2007).   DOI   ScienceOn
10 S. Shen, Z. Chang, J. Liu, X. Sun, X. Hu and H. Liu, Sep. Purification Tech., 53, 216-223(2007)   DOI   ScienceOn
11 J. Cinatl, B. Morgenstern, G. Bauer, P. Chandra, H. Rabenau and H. W. Doerr, Lancet, 361, 2045-2046 (2003)   DOI   ScienceOn
12 E. Choi, Biochem. Pharmacology, 70, 363-368(2005)   DOI   ScienceOn
13 A. R. Dehpour, M. E. Zolfaghari, T. Samadian, F. Kobarfard, M. Faizi and M. Assari, International J. Pharmaceutics, 119, 133-138(1995)   DOI   ScienceOn
14 J. Vaya, P. A. Belinky and M. Aviram, Free Radical Bio. Med., 23, 302-313(1997)   DOI   ScienceOn
15 T. Hatano, T. Yasubara and K. Miyamoto, Chem. Pharm. Bull., 36, 2286-2288(1988)   DOI   PUBMED
16 S. K. Acharya, S. Dasarathy, A. Tandon, Y. K. Joshi and B. N. Tandon, Indian J. Med. Res. B-Biomedical Research other than Infectious Diseases, 98, 69-74(1993)