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http://dx.doi.org/10.5806/AST.2006.19.6.529

The enhancement of protein separation by duplex SDS-PAGE  

Pyo, Jae Sung (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Roh, Si Hun (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Song, Jin-Su (Department of Chemistry, Seoul National University)
Lee, Kyung Hyeon (Department of Chemistry, Seoul National University)
Kim, Hie-Joon (Department of Chemistry, Seoul National University)
Park, Jeong Hill (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Kwon, Sung Won (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Publication Information
Analytical Science and Technology / v.19, no.6, 2006 , pp. 529-534 More about this Journal
Abstract
The protein separation with molecular weight using SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is the one of the most conventional and simple techniques. In, this study, two dimensional SDS-PAGE using same separation principle consecutively was investigated and compared with one dimensional SDS-PAGE. The enhanced separation from duplex SDS-PAGE was observed and separated proteins in the gel were identified by MALDI TOF MS. Identified proteins from different gel spots were found to have different gi numbers. Therefore, duplex SDS-PAGE separation method will be used for economic separation method in the future because only tiny amount of inexpensive reagents are used to perform duplex SDS-PAGE.
Keywords
protein separation; SDS-PAGE; MALDI TOF MS;
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