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http://dx.doi.org/10.5806/AST.2006.19.3.239

Determination of itraconazole in human plasma by high performance liquid chromatography  

Jang, Hae Jong (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.)
Lee, Ye Rie (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.)
Lee, Kyung Ryul (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.)
Han, Sang-Beom (College of Pharmacy, ChungAng University)
Kang, Seung Woo (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.)
Lee, Hee Joo (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.)
Publication Information
Analytical Science and Technology / v.19, no.3, 2006 , pp. 239-243 More about this Journal
Abstract
This method is used for the determination of itraconazole in human plasma by liquid-liquid extraction and high performance liquid chromatography. Felodipine was used as an internal standard. After extraction of the plasma with diethyl ether, the centrifuged upper layer was then transferred. The supernantant was evaporated and then reconsituted with mobile phase. The mobile phase was composed of 10 mM ammonium acetate adjusted to pH 7 by phosphoric acid with a flow rate of 0.2 mL/min. A C18 reversed-phase column with a pre-column was used as the analytial column. Linear detection responses were obtained for itraconzole concentration range for 2~1,000 ng/mL. The correlation coefficient of linear regression($R^2$) was 0.9991, limit of quantification (LOQ) was 2 ng/mL, reproducibility was less than 10.8 %, and accuracy was 97.2~108.2%. This method has been successfully applied to the pharmacokinetic study of itraconazole in human plasma.
Keywords
Itraconazole; Felodipine; High performance liquid chromatography;
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