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A Study of matrix metalloproteinase-9 inhibitor in root bark of ulmus davidiana planchon  

Kong, Kwang-Hoon (Department of Chemistry, Chung-Ang University)
Han, Kee-Jung (Department of Biochemistry, Medical School, Chung-Ang University)
Lee, Kwang-Soo (Department of Chemistry, Chung-Ang University)
Cho, Sung-Hye (Department of Chemistry, Chung-Ang University)
Publication Information
Analytical Science and Technology / v.18, no.2, 2005 , pp. 104-111 More about this Journal
Abstract
Several solvents were used to fractionate an extract obtained from the chapped root bark of Ulmus davidiana Planchon. The each fractionary part was condensed under reduced pressure and then examined to investigate the inhibitory effect on MMPs by modified gelatin zymography, where EA fraction showed the inhibition effect on the activity of MMPs. A compound showing inhibition effect on the MMPs was isolated and purified from EA fraction. Under IR, $^1H$- and $^{13}C$- NMR analyses it is very close to a catethin. This substance showed 48% inhibition effect on measurement of MMP-9 activity at 5 mM and 43% at 10 mM. To verify the effect of this substance on cells, human hepatoma, SK-Hep-1 cells as a cancer model, and Chang liver cells as a normal model were selected. MTT assay was performed to examine the cell viability by treatment of $1{\mu}L/mL$ of the purified substance on cells. The purified substance showed negligible toxicity on human liver cell line.
Keywords
Ulmus davidiana planchon; IR; NMR:MMPs; zymography; SK-hept-1; MTT;
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