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Development of the Discrimination Methods for Geographical Origin of Bracken(Pteridium aquilinum) by Capillary Electrophoresis  

Kim, Eun Young (Korea Food Research Institute)
Kim, Jung Hyun (Korea Food Research Institute)
Chung, Kyung Sook (Korea Food Research Institute)
Rhyu, Mee Ra (Korea Food Research Institute)
Publication Information
Analytical Science and Technology / v.17, no.2, 2004 , pp. 192-197 More about this Journal
Abstract
The discrimination of bracken (Korean vs. Chinese) has been attempted using capillary electrophoresis (CE). Bracken (Pteridium aquilinum) was extracted with 30% methanol and separated on a uncoated fused-silica ($50{\mu}m{\times}27cm$) capillary. Conditions for optimal analysis include: temperature, $40^{\circ}C$; voltage, 8 kV; and pressure injection time, 5 sec. The optimal separation buffer was 0.3 M borate buffer (pH 8.5) containing 40 mM CHAPS with 30% ethylene glycol. Under the optimal conditions established for CE, the ratio of specific peak area (peak PA-1) to other peak area (peak PA-2) was effective in discrimination of Korean and Chinese bracken. The mean accuracy for discrimination of Korean and Chinese brackens were 80% and 86%, respectively.
Keywords
bracken; capillary electrophoresis; conditions for optimal analysis; discrimination;
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