Browse > Article

Determination of Histidine by Spectrofluorometry  

Cha, Ki Won (Department of chemistry, Inha university)
Huang, Huazi (Department of chemistry, Inha university)
Publication Information
Analytical Science and Technology / v.17, no.2, 2004 , pp. 180-183 More about this Journal
Abstract
A simple and sensitive determination method of histidine has been investigated using the spectrofluorometry. An emission peak of $Eu^{3+}$ - TTA- histidine complex occurs at 470 nm in aquous solution with excitation at 235 nm and the fluorescence intensity increased linearly with addition of histidine in the range of $1{\times}10^{-7}-4{\times}10^{-6}M$. The detection limits was $5{\times}10^{-7}M$ with 3.5% RSD. The present method was applied to determine histidine content in the synthetic sample.
Keywords
Histidine; Amino acids; $Eu^{3+}$; TTA(thenoyltrifluoroacetone); EDTA(ethylenediaminetetracetic acid);
Citations & Related Records
연도 인용수 순위
  • Reference
1 K.Kurima, T. Sakano, Analytical science, 15, 489(1999).   DOI   ScienceOn
2 V.P. Hanko, J.S. Rohrer, Analytical Biochemistry, 324, 29(2004).
3 N. Tateda, K.Matsuhisa, K. Hasebe, N. Kitajima, J. chromatography B, 718, 235(1998).
4 F. Halvwrson, J.S. Bimen, J.R. Leta, J. Chem. Phys. 41, 157(1964).
5 S. Sato, M. Wada, Bull .Japan Chem. Soc. 43, 955(1970).
6 G.A. Crosby, R.E. Whan, R.M. Alire, J. Chem. Phys. 34, 744(1961).
7 H.Yu, Y. S. Ding, S. F. Mou, P. Jandik, J. Chromatography, A. 966, 89(2002).
8 K. W. Cha, C.I.Park, K.W. Park, Bull. Korean Chem. Soc. 23, 633(2002).
9 K. Hermann, D. Abeck, J. chromatography B, 749, 41-47(2000).
10 N. Tateda, K. Matsuhisa, K. Hasebe, T. Miura, Analytical science, 17, 775(2002).
11 Y. Ding, H. Yu, S.Mou, J. Chromatography, A.,982, 237(2002).