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Molecular Size Fractionation of Soil Fulvic Acid by Gel Filtration Chromatography and Analysis of Their Fluorescence Characteristics  

Chung, Kun-Ho (Nuclear Environment Research Division, Korea Atomic Energy Research Institute)
Shin, Hyun-Sang (Department of Environmental Engineering, Seoul National University of Technology)
Lee, Wanno (Nuclear Environment Research Division, Korea Atomic Energy Research Institute)
Cho, Yeong-Hyun (Nuclear Environment Research Division, Korea Atomic Energy Research Institute)
Choi, Geun-Sik (Nuclear Environment Research Division, Korea Atomic Energy Research Institute)
Lee, Chang-Woo (Nuclear Environment Research Division, Korea Atomic Energy Research Institute)
Publication Information
Analytical Science and Technology / v.17, no.2, 2004 , pp. 163-172 More about this Journal
Abstract
The molecular size distribution and fluorescence properties of soil fulvic acids (FA) were characterized by using gel filtration chromatography (GFC) and luminescence spectroscopy. The objectives of this work were to fractionate the FA extracted from a forest soil into different nominal molecular size using GFC system and to characterize the fluorescence properties (excitation, emission and synchronous) of these fractions using luminescence spectrometer. The GFC column was calibrated with polyethylene glycols, acetone and dextrane Blue. The total permeation volume of the GFC system was 404 mL and the void volume 130 mL. The GFC molecular weight of the soil FA was in the range of 190~8,900 Dalton and the molecular weight at the peak on the chromatogram was 930 Dalton. The fluorescence intensity ratio ($I_{498nm}/I_{390nm}$) was found to be increased with an increasing molecular weight. This results may suggest that the fulvic acid fractions with high molecular weight have large amount of the condensed aromatic compound.
Keywords
fulvic acids; GFC; molecular size fractionation; luminescence spectroscopy; fluorescence;
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