Quantitative Analysis of trans-Resveratrol in Red Wines by Solid Phase Extraction and LC/MS

In order to determine the contents of trans-resveratrol in red wine, which was mainly consumed in Korea, both LC/MS-ESI and LC/MS-APCI methods were used after solid-phase (Sep-Pak $C_{18}$-cartridges) extraction. The contents of trans-resveratrol obtained by LC/MS-ESI were detected in the range of $0.06-4.31{\mu}g/mL$. The recoveries were ranged from 88.4 to 97.9%. The values of relative standard deviation were ranged from 0.6 to 4.6% and the detection limit was $0.001{\mu}g/mL$. The contents of trans-resveratrol obtained by LC/MS-APCI were detected in the range of $0.09-4.02{\mu}g/mL$ and the detection limit was $0.005{\mu}g/mL$.

고체상 추출법과 LC/MS를 이용한 적포도주 중의 trans-Resveratrol 분석

적포도주에 들어 있는 trans-resveratrol을 분석하기 위하여 본 연구에서는 Sep-Pak $C_{18}$-cartridges를 이용한 고체상 추출법의 전처리 및 LC/MS-ESI 방법과 LC/MS-APCI 방법을 사용하여 국내에서 시판되고 있는 포도주에서 trans-resveratrol의 함량을 비교 분석하였다. LC/MS-ESI 방법에 의한 trans-resveratrol 함량은 $0.06-4.31{\mu}g/mL$로 검출되었고, 회수율은 88.4-97.9%, 상대 표준편차는 0.6-4.6%로 나타났으며, 검출한계는 $0.001{\mu}g/mL$이었다. LC/MS-APCI방법의 경우 trans-resveratrol 함량은 $0.09-4.02{\mu}g/mL$가 검출되었고, 검출한계는 $0.005{\mu}g/mL$로 나타났다.