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A study on the optimal HPLC condition for peptides complex analysis using mass spectrometry  

Kwon, Sung Won (The University of Texas Southwestern Medical Center at Dallas)
Park, Chul Hong (BIME Laboratories)
Publication Information
Analytical Science and Technology / v.16, no.1, 2003 , pp. 78-81 More about this Journal
Abstract
Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.
Keywords
liquid chromatography; mass spectrometry; peptides; proteomics;
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