Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Analysis and Prevalence of Azaspiracid Toxins in Imported Shellfish in Korea

국내 수입산 패류에서의 azaspiracid 독소 분석 및 발생수준

  • Hyojin Kwon (Department of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Seonghwan Moon (SGS Korea Co. Ltd.) ;
  • Sungeun Park (SGS Korea Co. Ltd.) ;
  • Seongwon Hwang (Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Daseul Yeo (Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Yuan Zhang (Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Danbi Yoon (Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Md. Iqbal Hossain (Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Gyung Jin Bahk (Department of Food and Nutrition, Kunsan National University) ;
  • Kwang-Sik Choi (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Jihyun Lee (Department of Food and Nutrition, College of Human Ecology, Seoul National University) ;
  • Changsun Choi (Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University)
  • 권효진 (중앙대학교 식품생명공학과) ;
  • 문성환 (한국에스지에스(주)) ;
  • 박성은 (한국에스지에스(주)) ;
  • 황성원 (중앙대학교 식품영양학과) ;
  • 여다슬 (중앙대학교 식품영양학과) ;
  • 장원 (중앙대학교 식품영양학과) ;
  • 윤단비 (중앙대학교 식품영양학과) ;
  • 이크발호세인 (중앙대학교 식품영양학과) ;
  • 박경진 (군산대학교 식품영양학과) ;
  • 최광식 (제주대학교 해양생명과학과) ;
  • 이지현 (서울대학교 식품영양학과) ;
  • 최창순 (중앙대학교 식품영양학과)
  • Received : 2024.08.23
  • Accepted : 2024.10.08
  • Published : 2024.10.30
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Abstract

Azaspiracids (AZAs) are a group of shellfish toxins that cause azaspiracid shellfish poisoning (AZP). They have been detected in mussels (Mytilus edulis) and oysters (Crassostrea gigas) in the Netherlands, Ireland, Italy, France, and the United Kingdom. In this study, we aimed to develop a method for the simultaneous detection of AZAs using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Simultaneous analyses of AZA-1, AZA-2, AZA-3, AZA-4, and AZA-5 were conducted according to AOAC ISO 17025 guidelines. Between 2020 and 2023, 467 imported shellfish were purchased from retail and wholesale markets in Korea. However, none of the samples tested positive for AZA-1, AZA-3, AZA-4, or AZA-5. Only two Arca avellana imported from Russia were positive for AZA-2 above the limit of detection (LOD) but below the limit of quantification (LOQ), with concentrations of 0.68 and 0.71 ng/g, respectively. This study found that the prevalence of AZA-2 was very low in shellfish imported into Korea.

본 연구는 국내에 수입되는 다양한 종류의 패류를 대상으로 미관리 독소인 AZA와 AZA유사체 4종에 대한 동시 검출법을 확립하였다. 2020-2023년 기간동안 수집된 수입 패류 467샘플을 대상으로 모니터링을 수행한 결과, 러시아에서 수입된 돌조개 2점에서 AZA-2가 정량한계 이하로 검출된 것을 확인하였다. 이는 해외의 AZA관리 기준에 미치치 않는 미량의 독소로 인체에 위해도는 없는 것으로 판단되었다. 본 연구에서 개발된 시험법으로 수입산 패류의 안전관리에 기여할 수 있을 것이다.

Keywords

Acknowledgement

본 연구는 식품의약품안전처 연구사업(20163MFDS641)의 지원을 받아서 수행되었다.

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