Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Determination of Tetrodotoxin and its Analog Composition in Korean Takifugu porphyreus Pufferfish

국내산 검복의 테트로도톡신 및 그 유사체 분석

  • Bong Ki Park (Department of Food Science and Technology, Chung-Ang University) ;
  • Hyunjun Lee (Department of Food Science and Technology, Chung-Ang University) ;
  • Jonghyub Park (Department of Food Science and Technology, Chung-Ang University) ;
  • Nobuhisa Kajino (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Kwang-Sik Choi (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Wan-Ok Lee (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Changsun Choi (Department of Food and Nutrition, Chung-Ang University) ;
  • Jihyun Lee (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
  • Received : 2024.09.24
  • Accepted : 2024.10.08
  • Published : 2024.10.30
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Abstract

Tetrodotoxin (TTX) is a potent neurotoxin responsible for pufferfish poisoning. The tissue distribution of TTX and its analogs in Takifugu porphyreus pufferfish sold in the Korean market was determined using ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometry in multiple reaction monitoring mode. The method detection limit for TTX was 0.006-0.009 mg/kg in high-fat and low-fat matrices. The intra-day (n = 5) and inter-day (3 days, n = 15) accuracy and precision results met the Codex guidelines. The ovaries of T. porphyreus pufferfish showed higher levels of TTX (up to 67.7 mg/kg) and its analogs compared to other tissues. Lower levels of TTX (non-detectable to 1.10 mg/kg) were found in the muscle and testis tissues, usually the edible parts of the pufferfish, than in other tissues. TrideoxyTTX was the major TTX analog across all tissues. This study contributes to the database of marine toxicity information on T. porphyreus species sold in Korea.

본 연구에서는 국내산 검복의 테트로도톡신 및 그 유사체를 UHPLC-QqQ MS를 이용하여 분석하였다. 테트로도톡신 분석법의 직선성, 검출 한계, 정량 한계, 정확성 및 정밀성을 확인한 뒤 국내산 검복의 부위별 테트로도톡신 함량을 분석한 결과 대부분의 시료에서 테트로도톡신이 검출되었으며 정소를 제외한 모든 부위에서 테트로도톡신이 검출되었다. 검복의 테트로도톡신 함량은 난소에서 0.27-67.7 mg/kg으로 가장 높았으며 껍질과 간에서도 가식 부위인 정소와 근육에 비해 높은 농도의 테트로도톡신이 검출되었다. 테트로도톡신 유사체의 경우, 국내산 검복에서 4,9-anhydroTTX, deoxyTTX, dideoxyTTX, trideoxyTTX, norTTX가 검출되었으며 이 중 trideoxyTTX는 국내산 검복의 모든 부위에서 주요 테트로도톡신 유사체로 확인되었다. 본 연구를 통해 국내산 검복의 테트로도톡신 및 그 유사체 함량 및 조성을 이해하고, 국내 해양생물 독소 연구에 해당정보가 활용될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 2024년도 식품의약품안전처의 연구개발비(20163MFDS641)로 수행되었으며 이에 감사드립니다.

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