Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Determination of Tetrodotoxin and its Analog Composition in Korean Farmed Takifugu rubripes Pufferfish

국내산 양식 자주복의 테트로도톡신 및 그 유사체 분석

  • Bong Ki Park (Department of Food Science and Technology, Chung-Ang University) ;
  • Hyunjun Lee (Department of Food Science and Technology, Chung-Ang University) ;
  • Jonghyub Park (Department of Food Science and Technology, Chung-Ang University) ;
  • Nobuhisa Kajino (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Kwang-Sik Choi (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Wan-Ok Lee (Department of Marine Life Science (BK21 FOUR) and Marine Science Institute, Jeju National University) ;
  • Changsun Choi (Department of Food and Nutrition, Chung-Ang University) ;
  • Jihyun Lee (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
  • Received : 2024.09.21
  • Accepted : 2024.10.08
  • Published : 2024.10.30
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Abstract

The tissue distribution of tetrodotoxin (TTX) and its analogs in farmed Takifugu rubripes pufferfish sold in Korean markets was determined using ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry in the multiple reaction monitoring mode. The method detection limit for TTX ranged from 0.019 to 0.022 mg/kg in high-fat and low-fat matrices. The intra-day (n = 5) and inter-day (3 d, n = 15) accuracy and precision results met the Codex guidelines. Tetrodotoxin was not detected in most of the analyzed tissue samples. In the farmed T. rubripes tissues, TTX levels reached a maximum of 0.07 mg/kg in the liver. Among the TTX analogs, dideoxyTTX, trideoxyTTX, and norTTX were detected in all samples. This study contributes to the database of marine toxicity information for farmed Takifugu species sold in Korean markets.

본 연구에서는 국내산 양식 자주복의 테트로도톡신 및 그 유사체를 UHPLC-QqQ MS를 이용하여 분석하였다. 테트로도톡신 분석법의 직선성과 일내 및 일간 정확도와 정밀성, 검출 한계, 정량 한계를 구한 결과 CODEX 가이드라인 기준에 적합하였다. 국내산 양식 자주복의 부위별 테트로도톡신 함량을 분석한 결과 일부 시료의 간과 근육 부위를 제외한 대부분의 시료에서 테트로도톡신이 검출되지 않았으며, 검출된 시료도 간과 근육 부위에서는 각각 0.07 mg/kg, 0.06 mg/kg의 낮은 농도로 테트로도톡신이 검출되었다. 테트로도톡신 유사체의 경우 국내산 양식 자주복에서 dideoxyTTX, trideoxyTTX, norTTX가 검출되었다. 이 중 dideoxyTTX는 국내산 양식 자주복의 모든 부위에서 주요 테트로도톡신 유사체로 확인되었다. 본 연구를 통해 국내산 양식 자주복의 테트로도톡신 및 그 유사체 분포를 이해하는데 도움을 줄 수 있으며 국내 해양생물 독소 데이터베이스 구축에 기여할 수 있을 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 2024년도 식품의약품안전처의 연구개발비(20163MFDS641)로 수행되었으며, 이에 감사드립니다.

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