Journal of Radiopharmaceuticals and Molecular Probes (대한방사성의약품학회지)

Korean Society of Radiopharmaceuticals and Molecular Probes (대한방사성의약품학회)
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Evaluation of [18F]AlF-ND-GUL for Imaging Prostate Cancer

  • Hye Yeon Seo (Department of Biomedical Sciences, Seoul National University Graduate School) ;
  • Nadeem Ahmed Lodhi (Isotope Production Division Pakistan Institute of Nuclear Science & Technology (PINSTECH)) ;
  • Jung Woo Byun (Department of Biomedical Sciences, Seoul National University Graduate School) ;
  • Ran Ji Yoo (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Jae Min Jeong (CellBion Co., Ltd) ;
  • Yun-Sang Lee (Department of Nuclear Medicine, Seoul National University Hospital)
  • Received : 2024.06.20
  • Accepted : 2024.06.28
  • Published : 2024.06.30
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Abstract

Prostate cancer (PCa) is the second most frequent cancer. Prostate specific membrane antigen (PSMA) is an effective target for the detection, staging and monitor the response of therapy in the management of PCa. In this study, we aimed to develop a 18F-labeled PSMA-PET tracer ([18F]AlF-ND-GUL) and test its feasibility for targeting PCa. A variety of labeling conditions was tested for optimization to achieve maximum radiolabeling efficiency. To test the imaging capacity of [18F]AlF-ND-GUL, in vivo PET/MR images were performed in 22Rv1 tumor bearing BALB/c nude mice after injection of radiotracer. Optimized labeling condition was to dissolve 300 nmol of precursor (ND-GUL) in buffer (pH 4, 0.1 M, NaOAc-HOAc) and react with [18F]AlF at 110℃ for 30 min. The [18F]AlF-ND-GUL maintained stability of >85% over 4 h. In-vivo molecular PET/MR imaging study results clearly showed high tumor uptake of [18F]AlF-ND-GUL over time. In conclusion, we optimized [18F]AlF-ND-GUL labeling conditions for PCa diagnosis and confirmed PSMA specific binding in vivo.

Keywords

Acknowledgement

이 성과는 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2021R1C1C2004706).

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