Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Comparison of Antioxidant Activity and Compounds by Purification Method of MD Pharmacopuncture

MD약침의 정제법별 항산화 활성 및 성분의 함량 비교

  • Received : 2024.09.17
  • Accepted : 2024.10.04
  • Published : 2024.10.31
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Abstract

Objectives MD pharmacopuncture are medicine developed for immune-related treatments, consisting of wild ginseng, Cordyceps militaris, and Amomum xanthoides. Due to the characteristics of the active ingredients of each herbal medicine, they are prepared using an extraction and purification method. We attempted to confirm standardization and efficacy for the proper development of MD pharmacopuncture. Methods Method validation was carried out to establish a method for analyzing the compounds of MD pharmacopuncture. To justify the purification process, the content of index compounds by high performance liquid chromatography and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl, 2,2-azino bis (3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power were measured and compared for each purification step. To measure the activity of MD pharmacopuncture, they were compared with wild ginseng pharmacopuncture prepared with wild ginseng using antioxidant activitys. Results The index compounds of MD pharmacopuncture were selected as cordycepin and ginsenoside Rg1. As purification was further carried out, the content of index compounds and activity increased, confirming the need for a purification process. Comparing the activity of wild ginseng pharmacopuncture and MD pharmacopuncture, it was confirmed that the activity of MD pharacopuncture was higher at most concentrations. Conclusions When developing a new herbal medicine prescription, research based on the three major elements of medicine-stability, safety, and efficacy-is needed to scientifically and standardize herbal medicine.

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