대한한의학회지 (The Journal of Korean Medicine)

  • 제45권3호
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  • Pages.40-53
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  • 2024
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  • 1010-0695(pISSN)
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  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
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이기거풍산의 급성독성 및 유전독성 평가

Acute toxicity and genotoxicity assessment of Ligigeopoong-san

  • 조성영 (한국한의약진흥원 한약비임상시험센터) ;
  • 황성민 (한국한의약진흥원 한약비임상시험센터) ;
  • 김수영 (한국한의약진흥원 한약비임상시험센터) ;
  • 김준섭 (한국한의약진흥원 한약비임상시험센터) ;
  • 우경완 (한국한의약진흥원 한약비임상시험센터) ;
  • 조현우 (한국한의약진흥원 한약비임상시험센터) ;
  • 노종현 (한국한의약진흥원 한약비임상시험센터)
  • Sung-Young Jo (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Sung-Min Hwang (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Su-Yeong Kim (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Jun-Sub Kim (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Kyeong-Wan Woo (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Hyun-Woo Cho (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Jong-Hyun Nho (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine)
  • 투고 : 2024.06.11
  • 심사 : 2024.08.19
  • 발행 : 2024.09.01
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초록

Objectives: This study aimed to investigate whether Ligigeopoong-san induces acute toxicity and genotoxicity Methods: Ligigeopoong-san contains the rhizome of Cnidium officinale MAKINO, an herb with potential teratogenicity. Teratogenicity is closely associated with genotoxicity. We analyzed whether Ligigeopoong-san induces acute toxicity and genotoxicity using various experimental models in accordance with Korean non-clinical test standards for pharmaceuticals and OECD test guidelines. Results : When Ligigeopoong-san was administered as a single dose to male and female rats, no toxic reactions, including organ damage, were observed at doses up to 2,500 mg/kg. In the bacterial reverse mutation test, no DNA mutations were detected at concentrations up to 5,000 ㎍/plate. In cell models, Ligigeopoong-san did not induce structural or numerical chromosomal aberrations at concentrations up to 2,000 ㎍/mL. Additionally, in animal studies, it did not cause bone marrow toxicity or form micronuclei in erythrocytes at doses up to 2,000 mg/kg. Conclusions : The experiments using various models demonstrated that Ligigeopoong-san did not induce acute toxicity or genotoxicity.

키워드

과제정보

본 연구는 보건복지부와 보건산업진흥원[RS-2023-KH139577], 한국생명기술연구조합의 과학기술혁신인재양성사업 지원을 받아 수행되었습니다.

참고문헌

  1. Park, M. J., Jin, S. R., Oh, J. H., Song, W. S., Lee, H. S., Woo, J. H., Hwang, K. H., Bae, G. H., & Yun, Y. C. (2022). Peripheral facial palsy due to cerebellar artery infarction is improved by Korean medical treatment: A case report. The Journal of Internal Korean Medicine, 43(2), 122-129. https://doi.org/10.22246/jikm.2022.43.2.122
  2. Sim, S. Y. (2015) Clinical research of Korean medical treatment for the peripheral facial paralysis. The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology, 28(4), 62-73. https://doi.org/10.6114/jkood.2015.28.4.062
  3. Lee, D. H., Kwon, B. I., Yu, J. S., Park, S. K., & Kim, J. H. (2022) Neural mechanisms underlying peripheral facial nerve palsy: A protocol for systematic review and meta-analysis of neuroimaging studies. Medicine, 101(48), e32110. https://doi.org/10.1097/MD.0000000000032110
  4. Wang, C. C., Li, L., Tang, L. Y., & Leung, P. C. (2012) Safety evaluation of commonly used Chinese herbal medicines during pregnancy in mice. Human Reproduction, 27(8), 2448-2456. https://doi.org/10.1093/humrep/des180
  5. Wang, H., Bao, Q., Yi, H., & Xia, Q. The evaluation of embryotoxicity of Ligusticum chuanxiong on mice and embryonic stem cells. Journal of Ethnopharmacology, 15;239, 111895. https://doi.org/10.1016/j.jep.2019.111895
  6. Choi, J. S., Han, J. Y., Koren, G., & Cho, Y. K. (2021) Evaluation of fetal and neonatal outcomes after ingestion of Cnidium root (Cnidium officinale Makino) during pregnancy. Early Human Development, 161, 105456. https://doi.org/10.1016/j.earlhumdev.2021.105456
  7. Mohamed, H. R. H., El-Atawy, R. H., Ghoneim, A. M., & El-Ghor, A. A. (2020) Induction of fetal abnormalities and genotoxicity by molybdenum nanoparticles in pregnant female mice and fetuses. Environmental Science and Pollution Research, 27(19), 23950-23962. https://doi.org/10.1007/s11356-020-08137-0
  8. Arguelles-Velazquez, N., Alvarez-Gonzalez, I., Madrigal-Bujaidar, E., & Chamorro-Cevallos, G. (2013) Amelioration of cadmium-produced teratogenicity and genotoxicity in mice given arthrospira maxima (Spirulina) treatment. Evidence-Based Complementary and Alternative Medicine, 2013, 604535. https://doi.org/10.1155/2013/604535
  9. Phillips, D. H., & Arlt, V. M. (2009) Genotoxicity: damage to DNA and its consequences. EXS, 99, 87-110. https://doi.org/10.1007/978-3-7643-8336-7_4
  10. De Assis, K. R., Ladeira, M. S., Bueno, R. C., Dos Santos, B. F., Dalben, I., & Salvadori, D. M. (2009) Genotoxicity of cigarette smoking in maternal and newborn lymphocytes. Mutation Research, 679(1-2), 72-78. https://doi.org/0.1016/j.mrgentox.2009.02.006 1016/j.mrgentox.2009.02.006
  11. Erhirhie, E. O., Ihekwereme, C. P., & Ilodigwe, E. E. (2018) Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdisciplinary Toxicology, 11(1), 5-12. https://doi.org/10.2478/intox-2018-0001
  12. Nair, A. B., & Jacob, S. (2016) A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7(2), 27-31. https://doi.org/10.4103/0976-0105.177703
  13. Hayashi, M. (2022) Opinion: regulatory genotoxicity: past, present and future. Genes and Environment, 44(1), 13. https://doi.org/10.1186/s41021-022-00242-5
  14. Levy, D. D., Zeiger, E., Escobar, P. A., Hakura, A., van der Leede, B. M., Kato, M., Moore, M. M., & Sugiyama, K. I. (2019) Recommended criteria for the evaluation of bacterial mutagenicity data (Ames test). Genetic Toxicology and Environmental Mutagenesis, 848, 403074. https://doi.org/10.1016/j.mrgentox.2019.07.004
  15. Sims, P., Grover, P. L., Swaisland, A., Pal, K., & Hewer, A. (1974) Metabolic activation of benzo(a)pyrene proceeds by a diol-epoxide. Nature, 252(5481), 326-328. https://doi.org/10.1038/252326a0
  16. Theisen, A., & Shaffer, L. G. (2010) Disorders caused by chromosome abnormalities. The Application of Clinical Genetic, 3, 159-174. https://doi.org/10.2147/TACG.S8884
  17. Kang, S. H., Kwon, J. Y., Lee, J. K., & Seo, Y. R. Recent advances in in vivo genotoxicity testing: prediction of carcinogenic potential using comet and micronucleus assay in animal models. Journal of Cancer Prevention, 18(4), 277-288. https://doi.org/10.15430/jcp.2013.18.4.277