The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Acute toxicity and genotoxicity assessment of Ligigeopoong-san

이기거풍산의 급성독성 및 유전독성 평가

  • Sung-Young Jo (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Sung-Min Hwang (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Su-Yeong Kim (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Jun-Sub Kim (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Kyeong-Wan Woo (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Hyun-Woo Cho (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine) ;
  • Jong-Hyun Nho (Korean Medicine Non-clinical Study Center, National Development Institute of Korean Medicine)
  • 조성영 (한국한의약진흥원 한약비임상시험센터) ;
  • 황성민 (한국한의약진흥원 한약비임상시험센터) ;
  • 김수영 (한국한의약진흥원 한약비임상시험센터) ;
  • 김준섭 (한국한의약진흥원 한약비임상시험센터) ;
  • 우경완 (한국한의약진흥원 한약비임상시험센터) ;
  • 조현우 (한국한의약진흥원 한약비임상시험센터) ;
  • 노종현 (한국한의약진흥원 한약비임상시험센터)
  • Received : 2024.06.11
  • Accepted : 2024.08.19
  • Published : 2024.09.01
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Abstract

Objectives: This study aimed to investigate whether Ligigeopoong-san induces acute toxicity and genotoxicity Methods: Ligigeopoong-san contains the rhizome of Cnidium officinale MAKINO, an herb with potential teratogenicity. Teratogenicity is closely associated with genotoxicity. We analyzed whether Ligigeopoong-san induces acute toxicity and genotoxicity using various experimental models in accordance with Korean non-clinical test standards for pharmaceuticals and OECD test guidelines. Results : When Ligigeopoong-san was administered as a single dose to male and female rats, no toxic reactions, including organ damage, were observed at doses up to 2,500 mg/kg. In the bacterial reverse mutation test, no DNA mutations were detected at concentrations up to 5,000 ㎍/plate. In cell models, Ligigeopoong-san did not induce structural or numerical chromosomal aberrations at concentrations up to 2,000 ㎍/mL. Additionally, in animal studies, it did not cause bone marrow toxicity or form micronuclei in erythrocytes at doses up to 2,000 mg/kg. Conclusions : The experiments using various models demonstrated that Ligigeopoong-san did not induce acute toxicity or genotoxicity.

Keywords

Acknowledgement

본 연구는 보건복지부와 보건산업진흥원[RS-2023-KH139577], 한국생명기술연구조합의 과학기술혁신인재양성사업 지원을 받아 수행되었습니다.

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