KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Plant Early Growth and Chlorophyll Fluorescence of Seven Varieties of Italian Ryegrass Under Various Salt Stress Conditions

다양한 염 스트레스 조건에서 이탈리안 라이그라스 7 품종의 초기 생장 및 엽록소 형광 반응

  • Ji-Hyeon Mun (Department of Crop Science and Biotechnology, Chonbuk National University) ;
  • In-Ha Lee (Department of Crop Science and Biotechnology, Chonbuk National University) ;
  • Ji-Young Shon (National Institute of Crop Sceince, RDA) ;
  • Nam-Jin Chung (Department of Crop Science and Biotechnology, Chonbuk National University)
  • 문지현 (전북대학교 농업생명과학대학 작물생명과학과) ;
  • 이인하 (전북대학교 농업생명과학대학 작물생명과학과) ;
  • 손지영 (농촌진흥청 국립식량과학원) ;
  • 정남진 (전북대학교 농업생명과학대학 작물생명과학과)
  • Received : 2024.08.04
  • Accepted : 2024.08.24
  • Published : 2024.09.01
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Abstract

This study evaluated the salt tolerance of Italian ryegrass varieties under various salt stress conditions. Seven varieties, including Kowineary, Greencall, Greenfarm, Greenfarm 3, IR 603, IR 605, and IR 901, were used in this experiment. Under various salt concentrations, there was no significant decrease compared to the control at salt concentrations of 0.1% to 0.3%. IR 901, Kowineary, and IR 605 had a lower relative reduction rate, but the absolute growth of IR 603 was the highest among the varieties. Among chlorophyll fluorescence parameters, ABS/RC, TRo/RC, ETo/RC, and DIo/RC values (activities expressed per reaction center) showed a significantly negative correlation with growth rates. In the experiment where the salt concentration was drastically increased, the growth decreases in Greenfarm 3 and IR 901 were relatively small compared to other varieties. Among the relative ratio of chlorophyll fluorescence parameters, ABS/RC, TRo/RC, ETo/RC (after 1 hour of salt concentration increase), and PIABS (3 hours, 7 days) showed a significant positive correlation, and DIo/RC (3 hours, 1 day) showed a negative correlation with plant growth. In the experiment where the salt concentration was drastically decreased, the growth increases in Greenfarm, IR 901, and Kowineary were relatively high compared to other varieties. Among the relative ratio of chlorophyll fluorescence parameters, ABS/RC, ETo/RC, DIo/RC (5 hours), and PIABS (1 hour) showed a significant positive correlation with plant growth. In summary, among the seven varieties, Kowineary and IR 901 had superior relative growth (compared to the control) under various salt stress conditions. IR 603 showed a greater decrease in relative growth but had the highest absolute growth amount under salt stress conditions. In addition, some parameters related to chlorophyll fluorescence showed a significant correlation with early growth, which could be used as indicators of the initial growth of IRG under salt stress.

본 연구는 염 스트레스 조건에서 이탈리안 라이그라스(IRG)의 엽록소 형광 반응을 검토하여 품종 간의 내염성을 평가하였다. 시험에 사용한 IRG 품종은 코윈어리, 그린콜, 그린팜, 그린팜 3호, IR 603, IR 605, IR 901등 7품종이다. 염농도에 따른 초기 생장은 온실에서 수경재배로 조사하였고, 염농도 변화 처리 시의 생육 및 엽록소 형광 시험은 생장상에서 수경재배로 조사하였다. 1. 염농도에 따른 품종별 초기 생장을 보면, 0.1%~0.3% 염농도 범위에서 코원어리, 그린콜, 그린팜, IR 605는 대조구와 유의한 차이가 없었으나, 그린팜 3호, IR 603, IR 901은 염농도 0.1%에서 오히려 대조구보다 생장량이 유의하게 증가하였다. 0.6% 이상의 염농도에서는 모든 품종의 생장량이 감소하였는데, 코윈어리, IR 605, IR 901 품종은 대조구에 비해 상대적인 감소 비율이 적었으며, IR 603의 감소 비율은 비교적 컸으나 절대적인 수량이 가장 많았다. 염농도에 따른 7품종의 엽록소 형광 측정 결과, 대부분의 품종이 0.1~0.3% 염농도의 OJIP 곡선에서 대조구와 유사하지만 염농도 0.6% 이상에서는 엽록소 형광량이 감소하였다. 엽록소 형광 파라미터 상대 비율 중 ABS/RC, TRo/RC, ETo/RC, DIo/RC 값은 식물체의 생장량과 유의하게 음의 상관관계를 보였다. 2. 염농도의 급격한 변화가 IRG에 미치는 영향을 검토한 결과, 염농도를 급격히 상승시킨 처리에서는 생장량, SPAD, 상대 수분 함량이 감소하고 전해질 용출량이 증가하였는데, 품종별로는 그린팜 3호와 IR 901품종의 변화가 비교적 적었다. 엽록소 형광 파라미터 상대 비율 중 염농도 상승 1시간 후의 ABS/RC, TRo/RC, ETo/RC, 염농도 상승 3시간 후와 7일 후의 PIABS는 식물체의 생장량과 유의한 양의 상관을 보였으며, 염농도 상승 3시간 후와 1일 후의 DIo/RC는 음의 상관을 보였다. 3. 염농도를 급격히 하강시킨 처리에서는 식물체의 생장량, SPAD, 상대 수분 함량이 증가하였고, 전해질 용출량이 감소하였는데, 품종별로는 그린팜, IR 901, 코윈어리 품종의 변화가 비교적 많았다. 엽록소 형광 파라미터 상대비율 중 염농도 하강 1시간 후의 PIABS, 5시간 후의 ABS/RC, ETo/RC, DIo/RC는 식물체의 생장량과 유의한 양의 상관을 보였다. 4. 이상의 결과를 종합해 보면, 염 스트레스와 염농도 변화 환경에서 IRG 7품종 중 코윈어리와 IR901 품종은 상대적 생장량이 우수하였다. 또한, IR603 품종은 염 스트레스 환경에서 생장량의 감소는 많지만, 다른 품종보다 절대적인 생장량이 많았다. 엽록소 형광 매개변수는 품종과 스트레스 환경에 따라 다르게 나타났으며, 이는 염 스트레스로 인한 반응중심의 활성 변화 때문일 것으로 보인다. 특히 염 스트레스를 판단하기 위한 생장 지표로, 반응중심 당 에너지의 흐름과 에너지 보존 효율을 의미하는 엽록소 형광 매개변수들이 효과적으로 이용될 것으로 판단된다.

Keywords

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