Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Leaf Spot Disease on White Chrysanthemum (Chrysanthemum morifolium) Caused by Epicoccum sorghinum in Korea

Epicoccum sorghinum에 의한 국화 점무늬병 발생

  • Sieun Kim (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jong-Hwan Shin (Research Institute of Climate Change and Agriculture, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Ha-Kyoung Lee (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Seong-Chan Lee (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • You-Kyoung Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 김시은 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 신종환 (농촌진흥청 국립원예특작과학원 온난화대응농업연구소) ;
  • 이하경 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 이성찬 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 한유경 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2024.07.21
  • Accepted : 2024.09.09
  • Published : 2024.09.30
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Abstract

In September 2023, a leaf spot disease occurred on chrysanthemum (Chrysanthemum morifolium) in Imsil-gun Agricultural Technology Center, Korea. A fungal species was isolated from the diseased plant and identified as an ascomycete Epicoccum sorghinum based on morphological characteristics and phylogenetic analyses using the sequences of the ITS, TUB2, and ACT genes. A pathogenicity test revealed that the isolate was the causal agent of leaf spot disease on chrysanthemum. This is the first report of leaf spot disease caused by E. sorghinum on chrysanthemum in Korea.

임실군농업기술센터에서 전시용으로 재배 중인 국화 잎에 갈색에서 검은색의 반점이 형성되고, 시간이 지날수록 병반이 확대되며 심하면 식물체가 고사하는 증상이 나타났다. 국화의 병든 잎 부위에서 병원균을 분리하여 ITS, TUB2, ACT 유전자 염기 서열 분석을 통해 동정한 결과 Epicoccum sorghinum으로 확인되었다. 분리된 균의 균학적 특성을 확인해 본 결과, PDA 배지에서 배양한 균을 UV 광에 12시간 동안 노출 후 암실에 두었을 때 병자각이 형성되었으며, 격벽이 없는 타원형의 무색 투명한 분생포자(3.2-6.0×1.7-3.0 ㎛)가 형성되는 것을 확인할 수 있었다. PDA 배지에서 배양한 균주의 agar plug를 국화 잎에 접종하여 병원성을 검정한 결과, 접종한 국화 잎에서 담갈색에서 검은색의 불규칙한 모양의 병반이 형성되었다. 따라서 E. sorghinum에 의한 국화 점무늬병을 국내 처음으로 보고하고자 한다.

Keywords

Acknowledgement

This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01509501), and supported by (2023) the RDA Fellowship Program of the National Institute of Horticultural & Herbal Science, Rural Development Administration, Republic of Korea.

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