The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Cestrum tomentosum L.f. Extracts against Colletotrichum scovillei by Altering Cell Membrane Permeability and Inducing ROS Accumulation

  • Guogeng Jia (Department of Applied Biology, Chungnam National University) ;
  • Sun Ha Kim (Department of Applied Biology, Chungnam National University) ;
  • Jiyoung Min (Department of Applied Biology, Chungnam National University) ;
  • Nelson Villalobos Zamora (Instituto Nacional de Biodiversidad (INBio)) ;
  • Silvia Soto Montero (Instituto Nacional de Biodiversidad (INBio)) ;
  • Soo-Yong Kim (International Biological Material Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Sang-Keun Oh (Department of Applied Biology, Chungnam National University)
  • Received : 2024.07.25
  • Accepted : 2024.08.20
  • Published : 2024.10.01
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Abstract

Chili pepper anthracnose, caused by Colletotrichum spp., is a significant biotic stress affecting chili fruits globally. While fungicide application is commonly used for disease management due to its efficiency and cost-effectiveness, excessive use poses risks to human health and the environment. Botanical fungicides offer advantages such as rapid degradation and low toxicity to mammals, making them increasingly popular for sustainable plant disease control. This study investigated the antifungal properties of Cestrum tomentosum L.f. crude extracts (CTCE) against Colletotrichum scovillei. The results demonstrated that CTCE effectively inhibited conidia germination and germ tube elongation at 40 ㎍/ml concentrations. Moreover, CTCE exhibited strong antifungal activity against C. scovillei mycelial growth, with an EC50 value of 18.81 ㎍/ml. In vivo experiments confirmed the protective and curative effects of CTCE on chili pepper fruits infected with C. scovillei. XTT analysis showed that the CTCE could significantly inhibit the cell viability of C. scovillei. Mechanistic studies revealed that CTCE disrupted the plasma membrane integrity of C. scovillei and induced the accumulation of reactive oxygen species in hyphal cells. These findings highlight CTCE as a promising eco-friendly botanical fungicide for managing C. scovillei infections in chili peppers.

Keywords

Acknowledgement

This work was supported by Project No. RS-2020-IP120086 of the Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET), Republic of Korea. The authors thank to the Comision Nacional para la Gestion de la Biodiversidad (CONAGEBIO) and the conservation area for the permission of the plant collection from the Amistad Pacifico Conservation Area under resolution RCM-INBio-168-2013-OT and RCM-INBio-170-2013-OT.

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