농업과학연구 (Korean Journal of Agricultural Science)

  • 제51권3호
  • /
  • Pages.361-373
  • /
  • 2024
  • /
  • 2466-2402(pISSN)
  • /
  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of processing methods and harvest timings for the efficient extraction of Syzygium formosum

  • Jaeyoon Lim (Department of Food and Nutrition, Chungnam National University) ;
  • Jaehan Kim (Department of Food and Nutrition, Chungnam National University) ;
  • In-Seok Yeo (Graduate School of Analytical Science and Technology, Chungnam National University) ;
  • Jong-Tae Park (Department of Food Science and Technology, Chungnam National University)
  • 투고 : 2024.07.29
  • 심사 : 2024.08.19
  • 발행 : 2024.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Syzygium formosum has been traditionally used in Vietnam as a folk medicine for treating skin and respiratory diseases. With the recent recognition of its beneficial effects such as anticancer, antioxidant, and anti-inflammatory properties, there is an increasing interest in industrial extraction of its bioactive compounds, such as triterpenoids and flavonoids. In this study, the extraction efficiencies of S. formosum were analyzed by extraction methods and harvest timings. Firstly, it was found that distilled water treatment substantially eliminated almost all flavonoids, suggesting that minimal pretreatment is important to preserve both triterpenoids and flavonoids. Secondly, extractions with 70% ethanol at both lab and pilot scales effectively yielded more than 70% of triterpenoids and flavonoids from S. formosum. Lastly, to investigate the seasonal variation in phytochemicals, extracts from the leaves of S. formosum harvested in April, August, October, and December in Vietnam were analyzed. It was revealed that seasons with lower temperatures led to higher concentrations of triterpenoids and flavonoids. Specifically, December was found to be optimal for obtaining high concentrations of triterpenoid and October for flavonoid. Consequently, this research provides a foundation for the industrial application of S. formosum, providing critical insights into maximizing the extraction efficiency and phytochemical contents through optimized processing methods and strategic harvesting.

키워드

과제정보

This work was supported by the research fund of Chungnam National University.

참고문헌

  1. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. 2017. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants 6:42. 
  2. Andre CM, Legay S, Deleruelle A, Nieuwenhuizen N, Punter M, Brendolise C, Cooney JM, Lateur M, Hausman JF, Larondelle Y, et al. 2016. Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids. New Phytologist 211:1279-1294. 
  3. Berini JL, Brockman SA, Hegeman AD, Reich PB, Muthukrishnan R, Montgomery RA, Forester JD. 2018. Combinations of abiotic factors differentially alter production of plant secondary metabolites in five woody plant species in the boreal-temperate transition zone. Frontiers in Plant Science 9:1257. 
  4. Bitwell C, Indra Sen S, Luke C, Kakoma MK. 2023. A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Scientific African 19:e01585. 
  5. Cai C, Ma J, Han C, Jin Y, Zhao G, He X. 2019. Extraction and antioxidant activity of total triterpenoids in the mycelium of a medicinal fungus, Sanghuangporus sanghuang. Scientific Reports 9:7418.
  6. Dinday S, Ghosh S. 2023. Recent advances in triterpenoid pathway elucidation and engineering. Biotechnology Advances 68:108214. 
  7. Ghosh S. 2016. Biosynthesis of structurally diverse triterpenes in plants: The role of oxidosqualene cyclases. Proceedings of the Indian National Science Academy 82:1189-1210. 
  8. Havsteen BH. 2002. The biochemistry and medical significance of the flavonoids. Pharmacology & Therapeutics 96:67-202. 
  9. Hoang KHT, Lim J, Nguyen MTT, Lee NY, Lee CK, Nguyen VD, Kim HL, Park JT, Kim J. 2021. Comparative phytochemical analysis of Syzygium formosum (Wall.) Masam leaf and its biological activities. Applied Science 11:10552. 
  10. Lee H, Oh IN, Kim J, Jung DH, Cuong NP, Kim Y, Lee J, Kwon O, Park SU, Lim Y, et al. 2018. Phenolic compound profiles and their seasonal variations in new red-phenotype head-forming Chinese cabbages. LWT 90:433-439. 
  11. Lee MH, Jiang CB, Juan SH, Lin RD, Hou WC. 2006. Antioxidant and heme oxygenase-1 (HO-1)-induced effects of selected Taiwanese plants. Fitoterapia 77:109-115. 
  12. Li Q, Lei S, Du K, Li L, Pang X, Wang Z, Wei M, Fu S, Hu L, Xu L. 2016. RNA-seq based transcriptomic analysis uncovers α-linolenic acid and jasmonic acid biosynthesis pathways respond to cold acclimation in Camellia japonica. Scientific Reports 6:36463. 
  13. Lin S, Zhang G, Liao Y, Pan J, Gong D. 2015. Dietary flavonoids as xanthine oxidase inhibitors: Structure-affinity and structure-activity relationships. Journal of Agricultural and Food Chemistry 63:7784-7794. 
  14. Loi DT. 2001. Medicinal Plants and Remedies of Vietnam. p. 826. Medical Publishing House, Hanoi, Vietnam. [in Vietnamese] 
  15. Ma B, Ren W, Zhou Y, Ma J, Ruan Y, Wen CN. 2011. Triterpenoids from the spores of Ganoderma lucidum. North American Journal of Medical Sciences 3:495-498. 
  16. Mansuri ML, Parihar P, Solanki I, Parihar MS. 2014. Flavonoids in modulation of cell survival signalling pathways. Genes & Nutrition 9:400. 
  17. Martin DE, Salzwedel K, Allaway GP. 2008. Bevirimat: A novel maturation inhibitor for the treatment of HIV-1 infection. Antiviral Chemistry and Chemotherapy 19:107-113. 
  18. Metodiewa D, Kochman A, Karolczak S. 1997. Evidence for antiradical and antioxidant properties of four biologically active N,N-diethylaminoethyl ethers of flavanone oximes: A comparison with natural polyphenolic flavonoid (rutin) action. Biochemistry and Molecular Biology International 41:1067-1075. 
  19. Misra RC, Sharma S, Sandeep, Garg A, Chanotiya CS, Ghosh, S. 2017. Two CYP716A subfamily cytochrome P450 monooxygenases of sweet basil play similar but nonredundant roles in ursane- and oleanane-type pentacyclic triterpene biosynthesis. New Phytologist 214:706-720. 
  20. Nguyen TMN, Lomunova M, Vu TPD, Le BV, Kim YH, Kang JS, Hwang, I. 2018. Anti-allergic effects of the ethanol extract of Syzygium formosum (Wall.) Masam leaves and its immunoregulatory mechanisms. Journal of Ethnopharmacology 211:171-179. 
  21. Park HA, Kim MY, Lee NY, Lim J, Park KB, Lee CK, Nguyen VD, Kim J, Park JT, Park JI. 2021. Variation of triterpenic acids in 12 wild Syzygium formosum and anti-inflammation activity on human keratinocyte HaCaT. Plants 10:2428. 
  22. Parul R, Das AK, Rana MS. 2020. Comparative pharmacological evaluation in respect to non-polar and polar solvent extracts of the leaves of Syzygium balsameum & Syzygium formosum. Journal of Pharmacognosy and Phytochemistry 9:45-52. 
  23. Phuong TT, Na M, Nguyen HD, Tran MH, Pham TK, Van Thanh T, Nguyen HN, Nguyen DT, Sok D, Bae, K. 2006. Antioxidant activities of Vietnamese medicinal plants. Natural Product Science 12:29-37. 
  24. Sandeep, Misra RC, Chanotiya CS, Mukhopadhyay P, Ghosh, S. 2019. Oxidosqualene cyclase and CYP716 enzymes contribute to triterpene structural diversity in the medicinal tree banaba. New Phytologist 222:408-424. 
  25. Shi Y, Ding Y, Yang, S. 2015. Cold signal transduction and its interplay with phytohormones during cold acclimation. Plant & Cell Physiology 56:7-15. 
  26. Thimmappa R, Geisler K, Louveau T, O'Maille P, Osbourn, A. 2014. Triterpene biosynthesis in plants. Annual Review of Plant Biology 65:225-257. 
  27. Uddin A, Hossain F, Reza A, Nasrin MS, Alam, A. 2022. Traditional uses, pharmacological activities, and phytochemical constituents of the genus Syzygium: A review. Food Science & Nutrition 10:1789-1819. 
  28. Wang GB, Cao FL, Chang L, Guo XQ, Wang, J. 2014. Temperature has more effects than soil moisture on biosynthesis of flavonoids in Ginkgo (Ginkgo biloba L.) leaves. New Forests 45:797-812. 
  29. Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang, Q. 2018. Response of plant secondary metabolites to environmental factors. Molecules 23:762.