Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Complete genome sequence of Treponema pedis GNW45 isolated from dairy cattle with active bovine digital dermatitis in Korea

  • Hector Espiritu (Department of Animal Science and Technology, Sunchon National University) ;
  • Lovelia Mamuad (Department of Animal Science and Technology, Sunchon National University) ;
  • Edeneil Jerome Valete (Department of Animal Science and Technology, Sunchon National University) ;
  • Sang-Suk Lee (Department of Animal Science and Technology, Sunchon National University) ;
  • Yong-Il Cho (Department of Animal Science and Technology, Sunchon National University)
  • Received : 2023.05.12
  • Accepted : 2023.06.25
  • Published : 2024.09.30
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Abstract

Treponema pedis, a fastidious anaerobic spirochete, is one of the main pathogens involved in the development and progression of bovine digital dermatitis (BDD), a lameness-causing hoof infection in cattle. Here, the complete genome sequencing of T. pedis GNW45 isolated from a dairy cow infected with BDD, was presented. Libraries for long and short reads were sequenced using PacBioRSII and Illimuna HiSeqXTen platforms, respectively. De-novo assembly was done using the long reads, producing a circular contig, by which the short reads were aligned to generate a more accurate genome sequence. The genome has a total size of 3,077,465 base pairs, with 36.84% guanine-cytosine content. A total of 2,749 protein-coding sequences, seven ribosomal RNA's, and 45 transfer RNA's were annotated. Functional analysis revealed genes associated with pathogenicity and survivability in the complex pathobiome of BDD. This study provided novel insights into the survival and pathogenic mechanisms of T. pedis GNW45.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF: Project No. 2021R1I1A3043691).

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