Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Rosa acicularis Leaves Exert Anti-Obesity Activity through AMPK-Dependent Lipolysis and Thermogenesis in Mouse Adipocytes, 3T3-L1 Cells

  • Jeong Won Choi (Department of Forest Science, Andong National University) ;
  • Hyeok Jin Choi (Department of Forest Science, Andong National University) ;
  • Jin Boo Jeong (Department of Forest Science, Andong National University)
  • Received : 2024.01.29
  • Accepted : 2024.02.13
  • Published : 2024.06.01
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Abstract

In this study, we aimed to verify the anti-obesity activity of R. acicularis leaves (RAL) and elucidate its mechanism of action in 3T3-L1 preadipocytes. RAL dose-dependently inhibited the accumulation of lipid droplets and triacylglycerol. RAL did not affect cell proliferation and survival in undifferentiated 3T3-L1 cells, but it inhibited cell proliferation in differentiating 3T3-L1 cells. RAL increased ATGL, p-HSL, and HSL, and decreased perilipin-1 in differentiating 3T3-L1 cells. In addition, RAL reduced lipid droplet accumulation and increased free glycerol content in differentiated 3T3-L1 cells. RAL increased ATGL and HSL in differentiated 3T3-L1 cells. Also, RAL increased p-AMPK, PPARγ, UCP-1, and PGC-1α in differentiating 3T3-L1 cells. AMPK inhibition by compound C attenuated RAL-mediated increase of ATGL, HSL, PPARγ, and UCP-1 in 3T3-L1 cells. Taken together, it is thought that RAL may inhibit lipid accumulation through lipolysis and thermogenesis via the activation of AMPK in adipocytes.

Keywords

Acknowledgement

This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) and funded by the Ministry of Education (NRF-2018R1A6A1A03024862).

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