The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Light- and Relative Humidity-Regulated Hypersensitive Cell Death and Plant Immunity in Chinese Cabbage Leaves by a Non-adapted Bacteria Xanthomonas campestris pv. vesicatoria

  • Young Hee Lee (Laboratory of Horticultural Crop Protection, Division of Horticultural Science, Gyeongsang National University) ;
  • Yun-Hee Kim (Laboratory of Plant Molecular Physiology, Department of Biology Education, Gyeongsang National University) ;
  • Jeum Kyu Hong (Laboratory of Horticultural Crop Protection, Division of Horticultural Science, Gyeongsang National University)
  • Received : 2024.03.26
  • Accepted : 2024.07.08
  • Published : 2024.08.01
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Abstract

Inoculation of Chinese cabbage leaves with high titer (107 cfu/ml) of the non-adapted bacteria Xanthomonas campestris pv. vesicatoria (Xcv) strain Bv5-4a.1 triggered rapid leaf tissue collapses and hypersensitive cell death (HCD) at 24 h. Electrolyte leakage and lipid peroxidation markedly increased in the Xcv-inoculated leaves. Defence-related gene expressions (BrPR1, BrPR4, BrChi1, BrGST1 and BrAPX1) were preferentially activated in the Xcv-inoculated leaves. The Xcv-triggered HCD was attenuated by continuous light but accelerated by a dark environment, and the prolonged high relative humidity also alleviated the HCD. Constant dark and increased relative humidity provided favorable conditions for the Xcv bacterial growth in the leaves. Pretreated fluridone (biosynthetic inhibitor of endogenous abscisic acid [ABA]) increased the HCD in the Xcv-inoculated leaves, but exogenous ABA attenuated the HCD. The pretreated ABA also reduced the Xcv bacterial growth in the leaves. These results highlight that the onset of HCD in Chinese cabbage leaves initiated by non-adapted pathogen Xcv Bv5-4a.1 and in planta bacterial growth was differently modulated by internal and external conditional changes.

Keywords

Acknowledgement

This work was financially supported by National Research Foundation (NRF) of Korea, Ministry of Education, Science and Technology (MEST) of Korea government (grant no. NRF-2020R1A2C1101613), Republic of Korea.

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