Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anti-Obesity Effect of Lactobacillus acidophilus DS0079 (YBS1) by Inhibition of Adipocyte Differentiation through Regulation of p38 MAPK/PPARγ Signaling

  • Youri Lee (Department of Microbiology, College of Medicine, Soonchunhyang University) ;
  • Navid Iqbal (Department of Microbiology, College of Medicine, Soonchunhyang University) ;
  • Mi-Hwa Lee (Nakdonggang National Institute of Biological Resources) ;
  • Doo-Sang Park (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yong-Sik Kim (Department of Microbiology, College of Medicine, Soonchunhyang University)
  • Received : 2024.02.07
  • Accepted : 2024.03.18
  • Published : 2024.05.28
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Abstract

Obesity is spawned by an inequality between the portion of energy consumed and the quantity of energy expended. Disease entities such as cardiovascular disease, arteriosclerosis, hypertension, and cancer, which are correlated with obesity, influence society and the economy. Suppression of adipogenesis, the process of white adipocyte generation, remains a promising approach for treating obesity. Oil Red O staining was used to differentiate 3T3-L1 cells for screening 20 distinct Lactobacillus species. Among these, Lactobacillus acidophilus DS0079, referred to as YBS1, was selected for further study. YBS1 therapy decreased 3T3-L1 cell development. Triglyceride accumulation and mRNA expression of the primary adipogenic marker, peroxisome proliferator-activated receptor gamma (PPARγ), including its downstream target genes, adipocyte fatty acid binding protein 4 and adiponectin, were almost eliminated. YBS1 inhibited adipocyte differentiation at the early stage (days 0-2), but no significant difference was noted between the mid-stage (days 2-4) and late-stage (days 4-6) development. YBS1 stimulated the activation of p38 mitogen-activated protein kinase (p38 MAPK) during the early stages of adipogenesis; however, this effect was eliminated by the SB203580 inhibitor. The data showed that YBS1 administration inhibited the initial development of adipocytes via stimulation of the p38 MAPK signaling pathway, which in turn controlled PPARγ expression. In summary, YBS1 has potential efficacy as an anti-obesity supplement and requires further exploration.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2015R1A6A103032522) and by the Korea Environmental Industry & Technology Institute (KEITI) through the Project to Make Multi-Ministerial National Biological Research Resources More Advanced funded by Korea Ministry of Environment (MOE) (2021003420003). This research was supported partially by a research fund of the Soonchunhyang University.

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