Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Preparation and Performance Evaluation of a Zinc Oxide-Graphene Oxideloaded Chitosan-Based Thermosensitive Gel

  • Hao Huang (Department of Prosthodontics, The Affiliated Hospital of Qingdao University) ;
  • Rui Han (Department of Prosthodontics, The Affiliated Hospital of Qingdao University) ;
  • Ping-Ping Huang (Department of Prosthodontics, The Affiliated Hospital of Qingdao University) ;
  • Chuan-Yue Qiao (Department of Prosthodontics, The Affiliated Hospital of Qingdao University) ;
  • Shuang Bian (Department of Prosthodontics, The Affiliated Hospital of Qingdao University) ;
  • Han Xiao (Department of Prosthodontics, The Affiliated Hospital of Qingdao University) ;
  • Lei Ma (Department of Prosthodontics, The Affiliated Hospital of Qingdao University)
  • Received : 2024.03.04
  • Accepted : 2024.04.09
  • Published : 2024.06.28
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Abstract

This study aimed to develop and assess a chitosan biomedical antibacterial gel ZincOxide-GrapheneOxide/Chitosan/β-Glycerophosphate (ZnO-GO/CS/β-GP) loaded with nano-zinc oxide (ZnO) and graphene oxide (GO), known for its potent antibacterial properties, biocompatibility, and sustained drug release. ZnO nanoparticles (ZnO-NPs) were modified and integrated with GO sheets to create 1% and 3% ZnO-GO/CS/β-GP thermo-sensitive hydrogels based on ZnO-GO to Chitosan (CS) mass ratio. Gelation time, pH, structural changes, and microscopic morphology were evaluated. The hydrogel's antibacterial efficacy against Porphyromonas gingivalis, biofilm biomass, and metabolic activity was examined alongside its impact (MC3T3-e1). The findings of this study revealed that both hydrogel formulations exhibited temperature sensitivity, maintaining a neutral pH. The ZnO-GO/CS/β-GP formulation effectively inhibited P. gingivalis bacterial activity and biofilm formation, with a 3% ZnO-GO/CS/β-GP antibacterial rate approaching 100%. MC3T3-e1 cells displayed good biocompatibility when cultured in the hydrogel extract.The ZnO-GO/CS/β-GP thermo-sensitive hydrogel demonstrates favorable physical and chemical properties, effectively preventing P. gingivalis biofilm formation. It exhibits promising biocompatibility, suggesting its potential as an adjuvant therapy for managing and preventing peri-implantitis, subject to further clinical investigations.

Keywords

Acknowledgement

This work was supported by the Clinical X Fund project of the Affiliated Hospital of Qingdao University Project (Project No.QDFY+X202101042).

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