Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Research Progress on Strategies for Improving the Enzyme Properties of Bacteriophage Endolysins

  • Yulu Wang (Shunde Women and Children's Hospital, Guangdong Medical University) ;
  • Xue Wang (Dongguan Key Laboratory of Public Health Laboratory Science, School of Public Health, Guangdong Medical University) ;
  • Xin Liu (Dongguan Key Laboratory of Public Health Laboratory Science, School of Public Health, Guangdong Medical University) ;
  • Bokun Lin (Shunde Women and Children's Hospital, Guangdong Medical University)
  • Received : 2024.01.02
  • Accepted : 2024.02.08
  • Published : 2024.06.28
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Abstract

Bacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, some methods have been used to improve the enzyme properties (bactericidal activity, lysis spectrum, stability and targeting the substrate, etc) of bacteriophage endolysins, including deletion or addition of domains, DNA mutagenesis, chimerization of domains, fusion to the membrane-penetrating peptides, fusion with domains targeting outer membrane transport systems, encapsulation, the usage of outer membrane permeabilizers. In this review, research progress on the strategies for improving their enzyme properties are systematically presented, with a view to provide references for the development of lysins with excellent performances.

Keywords

Acknowledgement

This work was supported by the Talents Recruitment Grant of Yangfan Plan of Guangdong Province (4YF16003G) and Dongguan Science and Technology of Social Development Program (20231800936342).

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