Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Effects of 2-Ethylhexyl Diphenyl Phosphate on the Development and Growth Hormone Endocrine System in Zebrafish Larvae

2-Ethylhexyl Diphenyl Phosphate가 제브라피쉬 치어의 발달과 성장호르몬 내분비계에 미치는 영향

  • Chaeun Park (Department of Environmental Health, Graduate School, Yongin University) ;
  • Da Yeong Lee (Department of Occupational and Environmental Health, Yongin University) ;
  • Kyunghee Ji (Department of Environmental Health, Graduate School, Yongin University)
  • 박채운 (용인대학교 일반대학원 환경보건학과) ;
  • 이다영 (용인대학교 산업환경보건학과) ;
  • 지경희 (용인대학교 일반대학원 환경보건학과)
  • Received : 2024.05.18
  • Accepted : 2024.06.12
  • Published : 2024.06.30
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Abstract

Background: 2-Ethylhexyl diphenyl phosphate (EHDPP) is widely used as a flame-retardant plasticizer in the production of polyvinyl chloride, adhesives, and food packaging. This chemical has been frequently detected in water, sediment, and indoor environments, and its lipophilicity raises concerns about bioaccumulation. Objectives: In this study, the effects of EHDPP on the development, behavioral changes, and growth hormone (GH) endocrine system of zebrafish larvae were investigated. Methods: Fertilized embryos were exposed to various concentrations (control, solvent control, 0.07, 0.7, 7, 70, and 700 ㎍/L) of EHDPP for 96 h. Developmental toxicity endpoints were observed daily. Behavioral changes under light-dark-light conditions and changes in hormones and genes related to GH/insulin-like growth factors (IGFs) axis were determined. Results: Significant decreases in survival, body length and moving distance were observed in zebrafish larvae exposed to 70 and 700 ㎍/L EHDPP. The concentrations of GH and IGF-1 were significantly decreased in zebrafish larvae exposed to 70 and 700 ㎍/L EHDPP. This change was well supported by changes in the transcription of genes involved in GH, IGF, IGF receptors, and IGF binding proteins. Conclusions: Our observations showed that exposure to 70 and 700 ㎍/L EHDPP could disrupt the feedback circuits of the GH/IGFs axis, ultimately leading to developmental toxicity, hypoactivity, and mortality.

Keywords

Acknowledgement

본 연구는 한국연구재단(RS-2023-00251751)의 지원을 받아 수행되었으며, 이에 감사드립니다.

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