DOI QR코드

DOI QR Code

Survey on the Occurrence of Five Grapevine Viruses in Korean Vineyards in 2021

2021년 국내 포도원에서의 5종 포도 바이러스 감염 실태 조사

  • Yeojin Choi (Department of Plant Medicine, Chungbuk National University) ;
  • Hae-Ryun Kwak (Crop Protection Division, National Institute of Agricultural Science) ;
  • Myung-Kyu Song (Chungbuk Agricultural Research and Extension Services) ;
  • Mikyeong Kim (Department of Plant Medicine, Chungbuk National University)
  • 최여진 (충북대학교 식물의학과) ;
  • 곽해련 (국립농업과학원) ;
  • 송명규 (충청북도 농업기술원) ;
  • 김미경 (충북대학교 식물의학과)
  • Received : 2024.01.11
  • Accepted : 2024.06.01
  • Published : 2024.06.30

Abstract

In this study, a total of 217 samples were collected from five grapevine cultivars in Chungcheongbuk-do and Gyeongsangbuk-do in 2021. These samples were tested for the presence of five grapevine viruses using specific primers through polymerase chain reaction. The results indicated that grapevine fleck virus was confirmed in 126 samples (58.06%), grapevine leafroll-associated virus (GLRaV)-3 in 112 samples (51.61%) and GLRaV-1 in four samples (1.84%). The infection rate was high in Shine Muscat and Alexandria cultivars, and by region, the Gyeongbuk region showed a high infection rate. However, the presence of grapevine red blotch associated virus and grapevine berry inner necrosis virus were not detected.

본 연구에서는 2021년 충청북도 및 경상북도에 위치한 7개 지역의 총 29개 포도 과원에서 재배되고 있는 포도 5개 품종에서 총 217의 시료를 채집하였다. 바이러스의 진단을 위해서 5종 바이러스에 대한 종 특이프라이머를 이용하여 중합효소연쇄반응하였다. 진단 결과 grapevine fleck virus (GFkV)는 126개 시료(58.06%)에서, grapevine leafroll-associated virus (GLRaV)-3는 112개 시료(51.61%)에서, GLRaV-1는 4개 시료(1.84%)에서 확인되었다. 특히 샤인머스캣과 알렉산드리아 품종에서의 감염률이 높았으며, 지역별로는 경북 지역이 높은 감염률을 보였다. 그러나 grapevine red blotch virus (GRBV)와 grapevine berry inner necrosis virus (GINV)는 확인되지 않았다.

Keywords

Acknowledgement

This research was supported by Chungbuk National University Korea National University Development Project (2021).

References

  1. Al Rwahnih, M., Dave, A., Anderson, M. M., Rowhani, A., Uyemoto, J. K. and Sudarshana, M. R. 2013. Association of a DNA virus with grapevines affected by red blotch disease in California. Phytopathology 103: 1069-1076. 
  2. Basso, M. F., Fajardo, T. V. M. and Saldarelli, P. 2017. Grapevine virus diseases: economic impact and current advances in viral prospection and management. Rev. Bras. Frutic. 39: e-411. 
  3. Cho, I. S., Chung, B. N., Hammond, J., Moon, J. S. and Lim, H. S. 2018. First report of grapevine rupestris vein feathering virus infecting grapevines in Korea. Plant Dis. 102: 1471. 
  4. Cho, I. S., Jung, S. M., Cho, J. D., Choi, G. S. and Lim, H. S. 2013. First report of grapevine pinot gris virus infecting grapevine in Korea. New Dis. Rep. 27: 10. 
  5. Cho, I. S., Yang, C. Y., Kwon, S. J., Yoon, J. Y., Kim, D. H., Choi, G. S. et al. 2019. First report of grapevine syrah virus 1 infecting grapevines in Korea. Plant Dis. 103: 2970. 
  6. Fan, X. D., Zhang, Z. P., Ren, F., Hu, G. J., Zhou, J., Li, Z. N. et al. 2017. Occurrence and genetic diversity of grapevine berry inner necrosis virus from grapevines in China. Plant Dis. 101: 144-149. 
  7. Fuchs, M. 2020. Grapevine viruses: a multitude of diverse species with simple but overall poorly adopted management solutions in the vineyard. J. Plant Pathol. 102: 643-653. 
  8. Jeong, S.-H., Kim, S.-H. and Heo, J.-Y. 2022. The first report of grapevine yellow speckle viroid 2 in Korea. J. Plant Pathol. 104: 891. 
  9. Jo, Y., Choi, H., Song, M. K., Park, J.-S., Lee, J. W. and Cho, W. K. 2017a. First report of grapevine yellow speckle viroid 1 and Hop stunt viroid infecting grapevines (Vitis vinifera) in Korea. Plant Dis. 101: 1069. 
  10. Jo, Y., Choi, H., Song, M. K., Park, J. S., Lee, J. W. and Cho, W. K. 2018a. First report of grapevine geminivirus A in diverse Vitis species in Korea. Plant Dis. 102: 255. 
  11. Jo, Y., Song, M.-K., Choi, H., Park, J.-S., Lee, J.-W. and Cho, W. K. 2017b. First report of grapevine rupestris stem pitting-associated virus in Grapevine in Korea. Plant Dis. 101: 1068. 
  12. Jo, Y., Song, M.-K., Choi, H., Park, J.-S., Lee, J.-W., Lian, S. et al. 2017c. Genome sequence of grapevine virus T, a novel foveavirus infecting grapevine. Genome Announc. 5: e00995-17. 
  13. Jo, Y., Song, M. K., Choi, H., Park, J. S., Lee, J. W. and Cho, W. K. 2017d. First report of grapevine fleck virus and grapevine virus E in grapevine in Korea. Plant Dis. 101: 1069. 
  14. Jo, Y., Song, M. K., Choi, H., Park, J. S., Lee, J. W. and Cho, W. K. 2017e. First report of grapevine fabavirus in diverse Vitis species in Korea. Plant Dis. 101: 1829.
  15. Jo, Y., Song, M. K., Choi, H., Park, J. S., Lee, J. W. and Cho, W. K. 2018b. First report of grapevine virus B in grapevine in Korea. Plant Dis. 102: 1466. 
  16. Kim, H. R., Lee, S. H., Lee, H. C., Kim, Y. T. and Park, J. W. 2004. Identification of grapevine leafroll-associated virus 3 ampelovirus from grapevines in Korea. Plant Pathol. J. 20: 127-130. 
  17. Kim, J.-S., Lee, S.-H., Choi, H.-S., Kim, M.-K., Kwak, H.-R., Nam, M. et al. 2011. Occurrence of virus diseases on major crops in 2010. Res. Plant Dis. 17: 334-341. (In Korean) 
  18. Kim, S.-H. and Heo, J.-Y. 2022. First report of grapevine virus L infecting grapevine in Korea. J. Plant Pathol. 104: 819. 
  19. Kim, S.-H, Jeong, S.-H. and Heo J.-Y. 2021. Incidence of 14 grapevine viruses in Korean vineyards. Not. Bot. Horti Agrobot. Cluj-Napoca. 49: 12490. 
  20. Korean Statistical Information Service (KOSIS). 2021. Status of main cultivars cultivated by outdoor grape farmers. ULR https://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1ET0014&conn_path=I2 [11 January 2024]. 
  21. Krenz, B., Thompson, J. R., McLane, H. L., Fuchs, M. and Perry, K. L. 2014. Grapevine red blotch-associated virus Is Widespread in the United States. Phytopathology 104: 1232-1240. 
  22. Kwak, H.-R., Yoon, J.-S., Shin, J.-C., Seo, J.-K., Kim, M., Lee, J.-K. et al. 2016. First report of blueberry leaf mottle virus on grapevine in Korea. Plant Dis. 100: 232. 
  23. Lim, S., Igori, D., Zhao, F., Moon, J. S., Cho, I.-S. and Choi, G.-S. 2016. First report of grapevine red blotch-associated virus on grapevine in Korea. Plant Dis. 100: 1957. 
  24. Martelli, G. P. 2017. An overview on grapevine viruses, viroids, and the diseases they cause. Grapevine viruses: molecular biology, diagnostics and management. Springer, Cham, Switzerland. 31-46 pp. 
  25. Myles, S., Boyko, A. R., Owens, C. L., Brown, P. J., Grassi, F., Aradhya, M. K. et al. 2011. Genetic structure and domestication history of the grape. Proc. Natl. Acad. Sci. U S A 108: 3530-3535. 
  26. This, P., Lacombe, T. and Thomas, M. R. 2006. Historical origins and genetic diversity of wine grapes. Trends Genet. 22: 511-519.