Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Microscopical observation and randomly amplified polymorphic DNA (RAPD) analysis of artificially cultivated Ganoderma applanatum

  • Woo-Sik Jo (Gyeongsangbuk-do Provincial Agricultural Research & Extension Service) ;
  • Young-Hyun Rew (Gyeongsangbuk-do Provincial Agricultural Research & Extension Service) ;
  • Seung-Chun Park (College of Veterinary Medicine, Kyungpook National University)
  • Received : 2024.06.18
  • Accepted : 2024.06.24
  • Published : 2024.06.30
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Abstract

This study investigated the microscopic characteristics and genetic relationships of Ganoderma applanatum fruiting bodies. Basidiospores were brown, ellipsoid, and had one or two large vacuoles and a double wall. The surface of basidiospores was smooth or wrinkled and most had numerous small and shallow holes. The length and width of basidiospores of Ganoderma applanatum isolates GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823, and ASI 53399 were on average 7.6×4.8 ㎛, 7.9×4.6 ㎛, 7.7×4.9 ㎛, 8.2×5.3 ㎛, 7.7×5.0 ㎛, 8.0×4.9 ㎛, and 7.9×4.9 mm, respectively. In contrast, the basidiospores of Ganoderma lucidum isolate ASI 7125 were 7.7×5.2 ㎛. Using the universal ITS1/ITS4 primer set, the ITS region of the isolates were amplified and sequenced. The ITS sequences were very closely related to G. applantum isolate GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823 and ASI 53399, but were not the same species. Whereas, G. lucidum isolate ASI 7125 belongs to different group.

Keywords

Acknowledgement

This study was conducted with the support of National Joint Agricultural Research Project of the RDA (Project No. 20080301-030-034-001-05-00), Republic of Korea.

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