Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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A study on the characteristics and pathogenicity of Aeromonas veronii isolated from infected goldfish (Carassius auratus)

피부 궤양이 발생한 금붕어(Carassius auratus)에서 분리한 Aeromonas veronii의 특성 및 병원성 분석

  • Hyeon Ki Jung (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Min Su Kim (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Sok Ho Kim (Department of Free Major, Kunsan National University) ;
  • Min Soon Choi (Department of Aquatic Life Medicine, Kunsan National University)
  • 정현기 (군산대학교 수산생명의학과) ;
  • 김민수 (군산대학교 수산생명의학과) ;
  • 김석호 (군산대학교 자율전공학부) ;
  • 최민순 (군산대학교 수산생명의학과)
  • Received : 2024.06.05
  • Accepted : 2024.06.15
  • Published : 2024.06.30
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Abstract

Aeromonas spp. infections have been reported to cause significant economic losses not only in the ornamental fish industry but also in aquaculture. In December 2022-January 2023, an Aeromonas infection occurred on a goldfish in korea, A gram-negative bacterium was isolated from the skin and internal organs of infected goldfish (Carassius auratus). The results showed that the isolate was identified as Aeromonas veronii using 16S rDNA targeted oilgpnucleotide primers, furthermore characteristics of A. veronii was confirmed by enterotoxin gene, infectious experiment, antibiotic resistance. In-vivo pathogenicity of isolates to goldfsh resulted in 100% mortality in challenged host within one week of post experiment injection. As a result of PCR analysis targeting three enterotoxin-encoding genes, cytotoxic enterotoxin (act) was identified in A. veronii isolate in this study. Antimicrobial susceptibility pattern of isolate showed it was to susceptible to most antimicrobial agents tested but resistant to ampicillin, imipenem, meropenem and clindamycin.

Aeromonas spp. 감염은 관상어 산업뿐만 아니라 양식업에서도 상당한 경제적 손실을 초래하는 것으로 보고되고 있다. 한편 2022년 12월부터 2023년 1월 사이에 국내에서 사육중인 금붕어(Crassius auratus)에서 Aeromonas병증을 보이는 어체에 대해서 발병 원인균을 규명하기 위해서 조사 하였다. 병증을 보이는 금붕어의 피부와 내부 장기에서 그람 음성균이 분리되었다. Biochemical test와 16S rRNA gene으로는 Aeromonas 속까지 동정할 수 있었으나 종레벨까지는 구분하기 어려웠다. 이에 가장 상동성이 높았던 A.veronii를 대상으로 species-specific PCR을 수행했고 명확히 종 수준으로 동정할 수 있었다. 아울러서 장독소 유전자, 감염 실험 및 항생제 내성 분석을 수행하였다. 금붕어에 대한 균주의 생체 내 병원성 시험 결과, 실험 주사 후 일주일 이내에 감염된 숙주에서 100%의 치사율을 나타냈다. 장독소 유전자인 act 독력 유전자가 검출되었다. 균주의 항균제 감수성 분석 결과 대부분의 항균제에 감수성을 보였으나, Ampicillin, Imipenem, Meropenem 및 Clindamycin 약제에는 내성을 보였다.

Keywords

Acknowledgement

이 논문은 2023년도 군산대학교 수산과학연구소 학술연구비 지원사업에 의하여 연구되었음.

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