대한본초학회지 (The Korea Journal of Herbology)

  • 제39권3호
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  • Pages.107-114
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  • 2024
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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선퇴 추출물의 Sprague-Dawley rat를 이용한 단회 경구 투여 독성시험

Single Dose Oral Toxicity Study of Cicadidae Periostracum Extracts in Sprague-Dawley Rats

  • 전병석 (농림축산검역본부 독성평가연구실) ;
  • 정희영 (전남대학교 수의과대학, BK21 Plus Project Team) ;
  • 이수은 (한국한의학연구원 한약자원연구센터) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터) ;
  • 김중선 (전남대학교 수의과대학, BK21 Plus Project Team) ;
  • 남현화 (한국한의학연구원 한약자원연구센터) ;
  • 이지혜 (부산대학교 한의학전문대학원)
  • Byung-Suk Jeon (Toxicological Evaluation Laboratory, Animal and Plant Quarantine Agency) ;
  • Huiyeong Jeong (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University) ;
  • Sueun Lee (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Yun-Soo Seo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Joong-Sun Kim (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University) ;
  • Hyeon Hwa Nam (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Ji Hye Lee (School of Korean Medicine, Pusan National University)
  • 투고 : 2024.04.24
  • 심사 : 2024.05.25
  • 발행 : 2024.05.30
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초록

Objective : Cicadae Periostracum (CP), which is the discarded shell of the Cryptotympana atrata (Fabricius, 1775), is a recognized component of oriental medicine for treatment sore throat, itching, shock, sedation, edema. However, the safety and toxicity of CP have not yet been established. It has been reported that symptoms of addiction or side effects may occur in patients who take high doses of CP or who are hypersensitive to it. Therefore, we investigated the acute toxicity of an CP extracts in Sprague-Dawley (SD) rats. Methods : To study acute toxicity, five SD rats of each sex per group were treated with CP extracts at single doses of 0, 500, 1000, or 2000 mg/kg administrated by oral gavage, and body weight, clinical signs, and mortality were observed after dosing. At the end of 14-day observation period, all animals were sacrificed and complete hematological and macroscopic examinations were performed. Results : There were no dead animal and test article-related effects on body weight change or the gross finding. No toxicologically significant results were observed between control and treated groups in hematology. Although salivation related to stress at the highest dose was observed in clinical signs immediately after administration, it is considered to have no toxicological significance. Conclusion : As the results, we did not find any adverse effect at the dose levels of 500, 1000, or 2000 mg/kg in rats. The minimal lethal dose was considered to be over 2000 mg/kg body weight in rats.

키워드

과제정보

This research was supported by grants for a Development of Sustainable Application for Standard Herbal Resources from the Korea Institute of Oriental Medicine, Republic Korea (KSN2012320) and Regional Innovation Strategy (RIS) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE)(2021RIS-002).

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