Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Network Pharmacological Analysis of Cnidii Fructus Treatment for Gastritis

벌사상자의 위염 치료 적용에 대한 네트워크 약리학적 분석

  • Young-Sik Kim (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Seungho Lee (Department of Pathology, College of Korean Medicine, Woosuk University)
  • 김영식 (우석대학교 한의과대학 본초학교실) ;
  • 이승호 (우석대학교 한의과대학 병리학교실)
  • Received : 2024.01.22
  • Accepted : 2024.02.08
  • Published : 2024.02.25
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Abstract

The purpose of this study was to identify the applicability, main compounds, and target genes of Cnidii Fructus (CF) in the treatment of gastritis using network pharmacology. The compounds in CF were searched in Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and a database of medicinal materials and chemical compounds in Northeast Asian traditional medicine (TM-MC). The target gene information of the compounds was collected from pubchem and cross-compared with the gastritis-related target gene information collected from Genecard to derive the target genes. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed on the derived target genes. Afterwards, network analysis between compounds and disease target genes was performed using cytoscape. We identified 121 active compounds and 139 target genes associated with gastritis. Pathways derived from the GO biological process and KEGG pathway DB primarily focus on target genes related to inflammation (IL-6, IL-8, TNF production, NF-κB transcription factor activity, and NF-κB signaling pathway) and cell death (PI3K-Akt, FoxO). Major targets for CF treatment of gastritis include TP53, TNF, BCL2, EGFR, NFKB1, ABCB1, PPARG, PTGS2, IL6, IL1B, and SOD1, along with major compounds such as coumarin, osthol, hexadecanoic acid, oleic acid, linoleic acid, and stigmasterol. This study provided CF's applicability for gastritis, related compounds, and target information. Evaluating CF's effectiveness in a preclinical gastritis model suggests its potential use in clinical practice for digestive system diseases.

Keywords

Acknowledgement

이 논문은 2021년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임(No.2021R1I1A3059594)

References

  1. Han SY, Kim YK. New approach for herbal formula research : network pharmacology. J. Physiol & Pathol Korean Med. 2016;30(6):385-96.
  2. Moncrieff J. Research on a "drug-centred" approach to psychiatric drug treatment: assessing the impact of mental and behavioural alterations produced by psychiatric drugs. Epidemiol Psychiatr Sci. 2018;27(2):133-40.
  3. Shay H, Komarov S, Fels S, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterol. 1945;5:43-61.
  4. Kim SG, Kim JG, Shin SK, Kim HS, Seol SY Guidelines of diagnosis for peptic ulcer disease. Korean J Gastroenterol. 2009;54:279-84.
  5. Health Insurance Review & Assessment Service. Healthcare Bigdata Hub. 2015 [cited 2024 Jan 10]. Available from. URL . http.//opendata.hira.or.kr/)
  6. Kim YS, Nam YS, Song JB, Kim HC. Gastroprotective and healing effects of Polygonum cuspidatum root on experimentally induced gastric ulcers in rats. Nutrients. 2020;12(8):2241.
  7. Herbology Editorial Committee of Korean Medicine School. Korean herbology (Boncho-hak). Seoul : Younglimsa. 2010.
  8. Li YM, Jia M, Li HQ, Zhang ND, Wen X, Rahman K, Zhang QY, Qin LP. Cnidium monnieri: A review of traditional uses, phytochemical and ethnopharmacological properties. Am J Chin Med. 2015;43(5):835-77.
  9. Korea Institute of Oriental Medicine. Defining dictionary for medicinal herbs [Korean, 'Hanyak giwon sajeon'](2024). Published on the Internet; https://oasis.kiom.re.kr/herblib/hminfo/hbmcod/hbmcod List.do (accessed 2024-01-03).
  10. Liu T, Zhang L, Joo D, Sun SC. NF-κB signaling in inflammation. Signal Transduct Target Ther. 2017;2:17023.
  11. Tong Y, Wang R, Liu X, Tian M, Wang Y, Cui Y, Zou W, Zhao Y. Zuojin pill ameliorates chronic atrophic gastritis induced by MNNG through TGF-β1/PI3K/Akt axis. J Ethnopharmacol. 2021;271:113893.
  12. Ucaryilmaz Metin C, Ozcan G. The HIF-1α as a potent inducer of the hallmarks in gastric cancer. Cancers (Basel). 2022;14(11):2711.
  13. Ito M, Tanaka S, Kim S, Kuwai T, Matsutani N, Kamada T, Kitadai Y, Sumii M, Yoshihara M, Haruma K, Chayama K. The specific expression of hypoxia inducible factor-1alpha in human gastric mucosa induced by nonsteroidal anti-inflammatory drugs. Aliment Pharmacol Ther. 2003;18 (Suppl. 3):90-2.
  14. Crabtree JE, Jeremy AH, Duval C, Dixon MF, Danjo K, Carr IM, Pritchard DM, Robinson PA. Effects of EGFR inhibitor on Helicobacter pylori Induced gastric epithelial pathology in vivo. Pathogens. 2013;2(4):571-90.
  15. Bellini MF, Cadamuro AC, Succi M, Proenca MA, Silva AE. Alterations of the TP53 gene in gastric and esophageal carcinogenesis. J Biomed Biotechnol. 2012;2012:891961.
  16. Vafaee R, Jahani Sherafat S, Rezaei Tavirani M, Ahmadi N. Down-regulation of TP53 is a highlighted molecular event in gastric ulcer. Gastroenterol Hepatol Bed Bench. 2022;15(3):249-55.
  17. Petryszyn PW, Wiela-Hojenska A. The importance of the polymorphisms of the ABCB1 gene in disease susceptibility, behavior and response to treatment in inflammatory bowel disease: A literature review. Adv Clin Exp Med. 2018;27(10):1459-63.
  18. Sun Y, Yang AWH, Lenon GB. Phytochemistry, ethnopharmacology, pharmacokinetics and toxicology of Cnidium monnieri (L.) Cusson. Int J Mol Sci. 2020;21(3):1006.
  19. Zhang ZR, Leung WN, Cheung HY, Chan CW. Osthole: A review on its bioactivities, pharmacological properties, and potential as alternative medicine. Evid Based Complement Alternat Med. 2015;2015:919616.
  20. Fan H, Gao Z, Ji K, Li X, Wu J, Liu Y, Wang X, Liang H, Liu Y, Li X, Liu P, Chen D, Zhao F. The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways. Phytomedicine. 2019;58:152864.
  21. Mandel KG, Bertram TA, Eichhold MK, Pepple SC, Doyle MJ. Fatty acid-mediated gastroprotection does not correlate with prostaglandin elevation in rats exposed to various chemical insults. Vet Pathol. 1994;31(6):679-88.