Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Monitoring of Residual Pesticides in Grape Seed Oil being Sold in Gyeonggi Province

경기도 내 판매되고 있는 포도씨유의 잔류농약 모니터링

  • Mi-Hui Son (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Jae-Kwan Kim (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • You-Jin Lee (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Ji-Eun Kim (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Eun-Jin Baek (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Byeong-Tae Kim (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Myoung-Ki Park (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment) ;
  • Bo-yeon Kwon (Ansan Agricultural and Fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment)
  • 손미희 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 김재관 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 이유진 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 김지은 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 백은진 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 김병태 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 박명기 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소) ;
  • 권보연 (경기도보건환경연구원 농수산물검사부 안산농수산물검사소)
  • Received : 2024.02.05
  • Accepted : 2024.03.22
  • Published : 2024.04.30
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Abstract

Using the freezing removal method, we investigated residual pesticides in 50 grape seed oils distributed in Gyeonggi Province, South Korea. The fat was mixed with acetonitrile and then frozen at ≤-20℃ for 24 h. Fats and oils were removed by separating those in solid state and the extract acetonitrile in liquid state. Ten residual pesticides were detected 161 times in 49 of 50 cases. The detected pesticides were boscalid, cyclufenamide, deltamethrin, difenoconazole, fluxapyroxad, fenpyrazamine, kresoxim-methyl, piperonyl butoxide, tebuconazole, and trifluoxysorbin. Boscalid, a fungicide, was most frequently detected (44 times), followed by fluxapiroxad (35 times). The detection range was 0.01-1.10 mg/kg, which was within the legal limit of residual pesticide for grapes. The recovery rate of the detected pesticides was 72.6-129.8% and the ratio of estimated daily intake/acceptable daily intake was calculated to determine the risk of the detected pesticides, which was <0.0028%. This indicated that the risk caused by pesticide residues in grape seed oil is at a safe level.

경기도에서 유통중인 포도씨유 50건을 대상으로 잔류농약 실태를 조사하였다. 50건 중 49건에서 10종의 잔류농약이 161회 검출되었다. 검출된 농약은 boscalid, cyflufenamid, deltamethrin, difenoconazole, fluxapyroxad, fenpyrazamine, kresoxim-methyl, piperonyl butoxide, tebuconazole, trifloxystrobin으로 살충제 2종, 살균제 8종이었으며 살균제인 boscalid가 44회, fluxapyroxad가 35회로 가장 빈번하게 검출되었다. 검출범위는 0.01-1.10 mg/kg으로 모두 포도의 잔류농약 허용 기준 이내로 검출되었다. 검출된 농약의 회수율은 72.6-129.8%이었으며 검출된 농약의 위해성을 알아보기 위해 %ADI를 산출한 결과 0.0028% 이하로 나타나 포도씨유의 잔류농약으로 인한 위해성은 안전한 수준으로 조사되었다. 그러나 본 연구는 시료의 수가 적고 GC/MS/MS로 분석가능한 농약만 조사하였기 때문에 보다 신뢰성 있는 결과를 얻기 위해선 앞으로도 꾸준한 추적 조사가 필요할 것으로 사료된다.

Keywords

References

  1. Park, K.S., Hur, Y.Y., Jung, S.M., No, J.H., Park, S.J., Hwang, H.S., (2023, December 4). Introduction to grape studies-from history to business administration. Retrieved from http://www.nongsaro.go.kr/portal/ps/psv/psvr/psvrc/rdaInterDtl.ps?menuId=PS00063&cntntsNo=34288 
  2. Rural Development Administration (RDA), (2023, December 4). Grape-farming skill guide. Retrieved from https://www.nongsaro.go.kr/portal/farmTechMain.ps?menuId=PS65291&stdPrdlstCode=FT040603 
  3. Kang, H.C., Lee, S.H., Kim, J.B., Quantification and physicochemical properties of grape seed lipids. J. Korean Soc. Agric. Chem. Biotechnol., 44, 173-178 (2001). 
  4. Hwang, J.T., Kang, H.C., Kim, T.S., Park, W.J., Lipid componant and properties of grape seed oils. Korean J. Food Nutr., 12, 150-155 (1999). 
  5. Kim, K.H., Anti-atopic effects and functional components of grape seed extract. PhD thesis, Chungbuk National University, Cheongju, Korea (2020). 
  6. Yoon, J,H., Antioxidants and physiological activities of grape seeds. Master's thesis, INJE University, Gimhae, Korea (2017). 
  7. Rural Development Administration (RDA), (2023, December 4). Control of major pests of grapes. Retrieved from http://www.nongsaro.go.kr/portal/ps/psb/psbo/vodPlay.ps?mvpNo=1824&mvpClipNo=&menuId=PS00069&cntntsNo=224780 
  8. Gyeonggi Provincial Health and Environment Research Institute, (2023, September 4). Gyeonggi province agricultural products residual pesticide statistical yearbook. Retrieved from https://www.gg.go.kr/opendata/openDataContent.do?gg_content_idx=8799&decodeIdx=5022 
  9. Food Information Statistics System (FIS), (2023, December 4). Status of processed food segment market in 2022(Edible oil). Retrieved from https://www.atfis.or.kr/home/board/FB0027.do?act=read&subSkinYn=N&bpoId=4384&bcaId=0&pageIndex=1 
  10. Moon, S.O., Lee, J.Y., Kim, E.J., Choi, S.W., An improved method for determination of catechin and its derivatives in extract and oil of grape seeds. Korean J. Food Sci. Technol., 35, 576-585 (2003). 
  11. Wie, M.J., Seong, J.H., Jeon, K.W., Jung, H.S., Lee, J.S., Comparison of Vitamin E, Phytosterols and fatty acid composition in commercially available grape seed oils in Korea. J. Korean. Soc. Food. Sci. Nutr., 37, 953-956 (2008).  https://doi.org/10.3746/jkfn.2008.37.7.953
  12. Jung, K.J., Shin, E.S., Kim, S.A., Quality characteristics of muffins with different fat and methods. Korean J. Food Cookery Sci., 24, 473-479 (2008). 
  13. An, Y.J., Analysis of functional constituents from the seed oils of gailiangmeoru and grapes. Master's thesis, Chung-Ang University, Seoul, Korea (2017). 
  14. He, Z., Wang Y., Wang, L., Peng, Y., Wang, W., Liu, X., Determination of 255 pesticides in edible vegetable oils using QuEChERS method and gas chromatography tandem mass spectrometry. Anal. Bio. Chem., 409, 1017-1030 (2017).  https://doi.org/10.1007/s00216-016-0016-9
  15. Li, L., Zhang, H., Pan, C., Zhou, Z., Jiang, S., Liu, F., Multiresidue analytical method of pesticides in peanut oil using low-temperature cleanup and dispersive solid phase extraction by GC-MS. J. Sep. Sci., 30, 2097-2104 (2007).  https://doi.org/10.1002/jssc.200700052
  16. Nguyen, T.D., Lee, M.H., Lee, G.H., Rapid determination of 95 pesticides in soybean oil using liquid-liquid extraction followed by centrifugation, freezing and dispersive solid phase extraction as cleanup steps and gas chromatography with mass spectrometric detection. Microchemical J., 95, 113-119 (2010).  https://doi.org/10.1016/j.microc.2009.11.009
  17. Li, L., Zhou, Z., Pan, C., Qian, C., Jiang, S., Liu, F., Determination of organophosphorus pesticides in soybean oil, peanut oil and sesame oil by low-temperature extraction and GC-FPD. Chromatographia, 66, 625-629 (2007).  https://doi.org/10.1365/s10337-007-0349-9
  18. Liu, Y., Shen, D., Mo, R., Zhong, D., Tang, F., Simultaneous determination of 15 multiresidue organophosphorous pesticides in camellia oil by MSPD-GC-MS. Bull. Environ. Contam. Toxicol., 90, 274-279 (2013).  https://doi.org/10.1007/s00128-012-0932-0
  19. Fuentes, E. Baez, M.E. Diaz, J., Microwave-assisted extraction at atmospheric pressure coupled to different cleanup methods for the determination of organophosphorus pesticides in olive and avocado oil. J. Chromatogr. A, 1216, 8859-8866 (2009).  https://doi.org/10.1016/j.chroma.2009.10.082
  20. Sanchez, A.G., Martos, N.R., Ballesteros, E., Multiresidue analysis of pesticides in olive oil by gel permeation chromatography followed by gas chromatography-tandem massspectrometric determination. Anal. Chim. Acta., 558, 53-61 (2006).  https://doi.org/10.1016/j.aca.2005.11.019
  21. Muhamad, H., Abdullah, M.P., Ai, T.Y., Chian, S.S., Optimization of the sweep co-distillation clean-up method for the determination of organochlorine pesticide residues in palm oil. J. Oil Palm Res., 16, 30-36 (2004). 
  22. Ministry of Food and Drug Safety (MFDS), Analytical practices manual for pesticide residues in foods, 6th ed, Cheongju, Korea, pp. 71-79 (2023). 
  23. Ministry of Food and Drug Safety (MFDS), (2023, December 4). Guidelines on standard procedures for preparing analysis method. Retrieved from http://www.nifds.go.kr/brd/m_15/view.do?seq=8215 
  24. International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH), (2023, September 4). Validation of analytical procedures: Text and methodology Q2(R1). Retrieved from https://database.ich.org/sites/default/files/Q2_R1__Guideline.pdf 
  25. Korea Health Industry Development Institute (KHIDI), (2023, December 4). Intake by food. Retrieved from https://www.khidi.or.kr/kps/dhraStat/result2?menuId=MENU01653&year=2020 
  26. Korean Statistical Information Service (KOSIS), (2023, December 4). Current status of average weight distribution by age and gender by city and province. Retrieved from https://kosis.kr/statHtml/statHtml.do?orgId=350&tblId=DT_35007_N132&vw_cd=MT_ZTITLE&list_id=350_35007_A007&conn_path=F0&path 
  27. Ministry of Food and Drug Safety (MFDS), (2023, December 4). Pesticides and veterinary drugs information. Retrieved from https://www.foodsafetykorea.go.kr/residue/prd/info/list.do?menuKey=1&subMenuKey=9 
  28. Ministry of Food and Drug Safety (MFDS), 2023. Pesticides MRLs in food, Cheongju, Korea, pp. 477-481. 
  29. National Pesticide Information Center (NPIC), (2023, December 4). Piperonyl butoxide general fact sheet. Retrieved from http://npic.orst.edu/factsheets/pbogen.pdf 
  30. Choi, S.H., Trans-generational toxicity of piperonyl butoxide in Daphnia magna, Master's thesis, University of Seoul, Seoul, Korea (2017). 
  31. Farajzadeh, M.A., Feriduni, B., Mogaddam, M.R.A., Determination of triazole pesticide residues in edible oils using air-assisted liquid-liquid microextraction followed by gas chromatography with flame ionization detection. J. Sep. Sci., 38, 1002-1009 (2015). https://doi.org/10.1002/jssc.201400818