한국치위생학회지 (Journal of Korean society of Dental Hygiene)

  • 제24권2호
  • /
  • Pages.131-139
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  • 2024
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  • 2287-1705(pISSN)
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  • 2288-2294(eISSN)
한국치위생학회 (Korean Society of Dental Hygiene)
권호 표지
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구강 세균 채취법에 따른 세균의 다양성과 양 분석을 위한 예비 연구

Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods

  • Seon-Ju Sim (Department of Dental Hygiene, Baekseok University) ;
  • Ji-Hye Kim (Department of Dental Hygiene, Baekseok University) ;
  • Hye-Sun Shin (Department of Dental Hygiene, Dongnam Health University)
  • 투고 : 2024.03.15
  • 심사 : 2024.04.12
  • 발행 : 2024.04.30
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초록

Objectives: Oral bacterial samples included subgingival, supragingival, and saliva plaques. As the diversity and number of microorganisms deffer depending on the area of the oral cavity and the method used, an appropriate and reliable collection method is important. The present study investigated oral bacterial sampling methods. Methods: Supragingival dental plaque was collected from the buccal and lingual tooth surfaces of study participants using sterilized cotton swabs. Plaques were collected from the subgingival area using a sterilized curette. Bacterial genomic DNA was extracted using MagNA Pure 96 DNA and Viral NA low-volume kits. Real-time polymerase chain reaction (PCR) was performed using the PowerCheckTM Periodontitis Pathogens Multiplex Real-time PCR kit. Results: Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Fusobacterium nucleatum of the orange complex were not observed in the subgingival biofilms of all study participants. For Porphyromonas. gingivalis, a significant correlation was observed between supragingival, subgingival, and total tooth surface biofilms. Compared to the supragingival and subgingival biofilmss, total tooth surface biofilm exhibited the highest bacterial count when the inswabbing method was used. Conclusions: Based on these findings, the supragingival swab method is recommended for oral bacterial research.

키워드

과제정보

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2022R1I1A1A01066197).

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