Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Neopestalotiopsis Leaf Blight, an Emerging Concern on Leatherleaf Fern in Indonesia

  • Ani Widiastuti (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Indah Khofifah Aruan (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Alvina Clara Giovanni (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Barokati Tsaniyah (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Tri Joko (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Achmadi Priyatmojo (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada)
  • Received : 2023.10.12
  • Accepted : 2023.12.26
  • Published : 2024.03.31
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Abstract

Leatherleaf fern (Rumohra adiantiformis) is an important ornamental plant in Indonesia and global. Green fern leaves with bold dark green color with long shelf-life, attract florists as decoration. Indonesia is one important leatherleaf fern exporters, however currently an outbreak of leaf blight decreased production significantly. Initial symptom was reddish brown spots from edge of leaf, which was gradually followed by dark-brown necrotic lesions causing leaf blight and dried. This is a study to do Koch-Postulate approach and molecular identification, to identify the pathogen of the "new emerging disease" reported. Based on multigene analysis using primers from ITS, β-tub and tef1-α gene markers, the pathogen was identified as Neopestalotiopsis sp. All sequences have been deposited in GenBank with accession number of OR905551 (ITS), OR899817 (ßtubulin) and OR899816 (TEF). This Neopestalotiopsis leaf blight causes an emerging concern in leatherleaf fern in Indonesia and global biosecurity because it infected an export commodity.

Keywords

Acknowledgement

Authors express the deepest gratitude to Universitas Gadjah Mada (UGM) Indonesia, which financially support this research through Academic Excellence 2023 Grant Universitas Gadjah Mada Number 7725/UN1.P.II/Dit-Lit/PT.01.03/2023. Authors also deeply thank PT. Tropika Flora Persada, Magelang, Central Java, Indonesia for providing leatherleaf fern field for disease observation, sampling and healthy leaves for pathogenicity test. Authors also thank Dr. Adyatma Irawan Santosa for assistance in bioinformatics and phylogenetic tree analysis.

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