DOI QR코드

DOI QR Code

First Report of Lasiodiplodia theobromae Causing Gummosis on Citrus grandis (L.) Osbeck in Vietnam

  • Vo Thi Ngoc Trai (Laboratory of Enzyme and Protein Technology, Institute of Biotechnology, Hue University) ;
  • Tran Thi Thu Ha (Department of Plant Protection, University of Agriculture and Forestry, Hue University) ;
  • Nguyen Bao Hung (Department of Applied Biology and Biotechnology, Institute of Biotechnology, Hue University)
  • Received : 2023.11.23
  • Accepted : 2024.03.15
  • Published : 2024.03.31

Abstract

This study aims to isolate and identify the fungal pathogen responsible for gummosis disease affecting Thanh Tra pomelo in Vietnam. Through molecular identification utilizing primer pairs ITS5 and ITS4, the analysis pinpointed Lasiodiplodia theobromae as the specific fungal pathogen. Notably, the fungal colonies exhibited vigorous growth on potato dextrose agar. Initially, these colonies appeared whitish-grey, transforming into a black-grey hue within 5-7 days at a temperature of 30℃. According to previous reports, Phytophthora spp. was the most common pathogenic genus causing gummosis on Thanh Tra pomelo in Vietnam. To our knowledge, this is the first report on L. theobromae causing gummosis on Thanh Tra pomelo in Vietnam.

Keywords

Acknowledgement

This study was carried out with the support of project belong to the Science and Technology Program at Ministerial level (Project No. CT2022.09.DHH.05), Ministry of Education and Training, Vietnam.

References

  1. Dwiastuti, M. E. and Aji, T. G. 2021. Citrus stem rot disease (Lasiodiplodia theobromae (Pat.) Griff. & Maubl) problem and their control strategy in Indonesia. IOP. Conf. Ser.: Earth Environ. Sci. 752:012030.
  2. Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. 1990. PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA, USA. 315-322 pp.
  3. Khanzada, M. A., Lodhi, A. M. and Shahzad, S. 2004. Pathogenicity of Lasiodiplodia theobromae and Fusarium solani on mango. Pak. J. Bot. 36: 181-189.
  4. Munirah, M. S., Azmi, A. R., Yong, S. Y. C. and Nur Ain Izzati, M. Z. 2017. Characterization of Lasiodiplodia theobromae and L. pseudotheobromae causing fruit rot on pre-harvest mango in Malaysia. Plant Pathol. Quar. 7: 202-213. https://doi.org/10.5943/ppq/7/2/14
  5. Pham, T. D., Nguyen, T. B. N., Cai, V. T., Nguyen, L., Nguyen, N. D., Do, D. H. et al. 2015. Effect of some fungicides to control gummosis caused by Phytophthora citrophthora on Thanh tra pummelo in Thua Thien Hue. J. Vietnam Agr. Sci. Tech. 01: 53-59.