Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Preservation of Simmental bull sperm at 0℃ in Tris dilution: effect of dilution ratio and long-distance transport

  • Shouqing Jiang (College of Animal Science and Technology, Tarim University) ;
  • Fei Huang (College of Life Science and Technology, Tarim University) ;
  • Peng Niu (College of Animal Science and Technology, Tarim University) ;
  • Jieru Wang (College of Life Science and Technology, Tarim University) ;
  • Xiaoxia He (College of Animal Science and Technology, Tarim University) ;
  • Chunmei Han (College of Animal Science and Technology, Tarim University) ;
  • Qinghua Gao (College of Animal Science and Technology, Tarim University)
  • Received : 2023.06.23
  • Accepted : 2023.08.28
  • Published : 2024.02.01
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Abstract

Objective: This study aimed to assess the impact of the dilution ratio of Tris diluent, storage at 0℃, and long-distance transportation on the spermatozoa of Simmental cattle. It also validated the feasibility of the regional distribution of fresh semen. Methods: In experiment 1, semen was diluted at four dilution ratios (1:6, 1:9, 1:12, and 1:15) to determine the optimal dilution ratio of Tris diluent. In experiment 2, we assessed sperm viability, progressive motility (objectively assessed by computer-assisted sperm analyzer), and acrosome intactness in Tris dilutions kept at constant 0℃ for 1, 3, 6, 9, and 12 days. We compared them to Tianshan livestock dilutions (Commercial diluent). In experiment 3, semen was diluted using Tris diluent, and sperm quality was measured before and after long-distance transport. Artificial insemination of 177 Simmental heifers compared to 156 using Tianshan Livestock dilution. Results: The outcomes demonstrated that 1:9 was the ideal Tris diluent dilution ratio. The sperm viability, Progressive Motility, and acrosome integrity of both Tris and Tianshan dilutions preserved at 0℃ gradually decreased over time. sperm viability was above 50% for both dilutions on d 9, with a flat rate of decline. The decrease in acrosome integrity rate was faster for Tianshan livestock dilutions than for Tris dilutions when stored at 0℃ for 1 to 6 days. There was no significant difference (p>0.05) in sperm viability between semen preserved in Tris diluent after long-distance transportation and semen preserved in resting condition. The conception rates for Tris dilution and Tianshan livestock dilution were 49.15% and 46.15% respectively, with no significant difference (p>0.05). Conclusion: This shows that Tris diluent is a good long-term protectant. It has been observed that fresh semen can be successfully preserved for long-distance transport when stored under 0℃ conditions. Additionally, it is feasible to distribute semen regionally.

Keywords

Acknowledgement

We are grateful for the support of Tarim University, China Dingxin Seed Technology Co., and the Livestock Workstation of Qiemo County, China.

References

  1. Verberckmoes S, Soom A, Dewulf J, Kruif A. Comparison of three diluents for the storage of fresh bovine semen. Theriogenology 2005;63:912-22. https://doi.org/10.1016/j.theriogenology.2004.05.011
  2. Yang DH, Standley NT, Xu ZZ. Application of liquid semen technology under the seasonal dairy production system in New Zealand. Anim Reprod Sci 2018;194:2-10. https://doi.org/10.1016/j.anireprosci.2018.01.004
  3. Pezo F, Romero F, Zambrano F, Sanchez RS. Preservation of boar semen: An update. Reprod Domest Anim 2019;54:423-34. https://doi.org/10.1111/rda.13389
  4. Campbell RC, Edwards J. Semen diluents in the artificial insemination of cattle. Nature 1954;173:637. https://doi.org/10.1038/173637a0
  5. Schulze M, Bortfeldt R, Schafer J, Jung M, Fuchs-Kittowski F. Effect of vibration emissions during shipping of artificial insemination doses on boar semen quality. Anim Reprod Sci 2018;192:328-34. https://doi.org/10.1016/j.anireprosci.2018.03.035
  6. Yates JH, Chandler JE, Canal AL, et al. The effect of nocturnal sampling on semen quality and the efficiency of collection in bovine species. Theriogenology 2003;60:1665-77. https://doi.org/10.1016/s0093-691x(03)00175-4
  7. Lone S, Mohanty T, Bhakat M, et al. Effect of over dilution of semen with tris extender on motion and functional attributes of bull spermatozoa during cryopreservation. Andrologia 2022;54:e14478. https://doi.org/10.1111/and.14478
  8. Qu Y, Chen Q, Guo S, et al. Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization. Protein Cell 2021;12:810-7. https://doi.org/10.1007/s13238-021-00825-y
  9. Pizzari T, Foster KR. Sperm sociality: cooperation, altruism, and spite. PLoS Biol 2008;6:e130. https://doi.org/10.1371/journal.pbio.0060130
  10. Fisher HS, Giomi L, Hoekstra HE, et al. The dynamics of sperm cooperation in a competitive environment. Proc R Soc B 2014;281:20140296. https://doi.org/10.1098/rspb.2014.0296
  11. Hayden S, Blanchard T, Brinsko S, et al. The "dilution effect" in stallion sperm. Theriogenology 2015;83:772-7. https://doi.org/10.1016/j.theriogenology.2014.11.012
  12. Harrison RAP, Dott HM, Foster GC. Bovine serum albumin, sperm motility, and the "dilution effect". J Exp Zool 1982;222:81-8. https://doi.org/10.1002/jez.1402220111
  13. Zhao JQ, Xiao GL, Zhu WL, et al. Ram semen preserved at 0℃ with soybean lecithin Tris-based extender substituted for egg yolk. Anim Biosci 2021;34:192-7. https://doi.org/10.5713/ajas.20.0118
  14. Vishwanath R, Shannon P. Storage of bovine semen in liquid and frozen state. Anim Reprod Sci 2000;62:23-53. https://doi.org/10.1016/s0378-4320(00)00153-6
  15. Andrade AFC, Grossfeld R, Knox RV. In vitro effects of two different commercial freezing and thawing extenders on boar sperm quality. Anim Reprod Sci 2022;236:106906. https://doi.org/10.1016/j.anireprosci.2021.106906
  16. Zhang B, Wang Y, Wu C, et al. Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS. BMC Vet Res 2021;17:127. https://doi.org/10.1186/s12917-021-02804-1
  17. Aitken RJ, Drevet JR, Moazamian A, Gharagozloo P. Male Infertility and oxidative stress: a focus on the underlying mechanisms. Antioxidants (Basel, Switzerland) 2022;11:306. https://doi.org/10.3390/antiox11020306
  18. Katherine M, Roslyn B, Gareth E, et al. Cryopreservation of epididymal alpaca (Vicugna pacos) sperm: a comparison of citrate-, Tris- and lactose-based diluents and pellets and straws. Reprod Fertil Dev 2007;19:792-6. https://doi.org/10.1071/rd07049
  19. Agarwal S, Kharche SD, Bhatiya AK. Study of TALP and TRIS citrate medium on caprine sperm capacitation and subsequent in vitro embryo production. Iranian J Vet Res 2017;18:275-8.
  20. Brown-Woodman PD, Post EJ, Chow PY, et al. Effects of malonic, maleic, citric and caffeic acids on the motility of human sperm and penetration of cervical mucus. Int J Fertil 1985;30:38-44.
  21. Pei-Pei Z, Jing-Jing W, Chong-Yang L, et al. Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress. Reprod Domest Anim 2020;55:1103-14. https://doi.org/10.1111/rda.13748
  22. Karimipour M, Dibayi Z, Ahmadi A, Javanmard MZ, Hosseinalipour E. The protective effect of vitamin C on phenylhydrazine-induced hemolytic anemia on sperm quality and in-vitro embryo development in mice. Int J Reprod Biomed 2018;16:12. https://doi.org/10.18502/ijrm.v16i12.3685
  23. Paschoal A, Luther A, Jakop U, Schulze M, Bortolozzo FP, Waberski D. Factors influencing the response of spermatozoa to agitation stress: Implications for transport of extended boar semen. Theriogenology 2021;175:54-60. https://doi.org/10.1016/j.theriogenology.2021.08.033
  24. Ideta A, Sakai S, Nakamura Y, et al. Administration of peripheral blood mononuclear cells into the uterine horn to improve pregnancy rate following bovine embryo transfer. Anim Reprod Sci 2010;117:18-23. https://doi.org/10.1016/j.anireprosci.2009.04.004
  25. Oliveira LZ, Arruda RP, Andrade AFC, et al. Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program. Anim Reprod Sci 2013;137:145-55. https://doi.org/10.1016/j.anireprosci.2013.01.010
  26. Parinaud J, Mieusset R, Vieitez G, Labal B, Richoilley G. Influence of sperm parameters on embryo quality. Fertil Steril 1993;60:888-92. https://doi.org/10.1016/s0015-0282(16)56292-x