Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Development of microsatellite markers to assess the genetic diversity of the red-tongue viper, Gloydius ussuriensis (Reptilia: Viperidae) on the Korean Peninsula

  • Jung A Kim (National Institute of Biological Resources) ;
  • Mu-Yeong Lee (DNA Analysis Division, Seoul Institute National Forensic Service) ;
  • Hye Sook Jeon (National Institute of Biological Resources) ;
  • Min Seock Do (National Institute of Biological Resources) ;
  • Kyo Soung Koo (Research Institute of EcoScience, Ewha Womans University) ;
  • Sang-Cheol Lee (Division of Life Sciences, Incheon National University) ;
  • Ji-Hwa Jung (Division of Zoology, Honam National Institute of Biological Resources) ;
  • Yoon-Jee Hong (National Institute of Biological Resources) ;
  • Junghwa An (National Institute of Biological Resources)
  • Received : 2023.04.12
  • Accepted : 2023.09.01
  • Published : 2023.11.30
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Abstract

The red-tongue viper(Gloydius ussuriensis) is one of only three species of the genus Gloydius found in South Korea. Gloydius ussuriensis has a narrow activity radius and is distributed nationwide, and this species was reported to have the largest population among the Korean species in genus Gloydius. Preliminary results of a phylogenetic analysis using part of the mitochondrial DNA indicated that domestic G. ussuriensis is not comprised of monophyletic groups, and morphological analysis showed differences between domestic populations. In this study, we developed 17 microsatellites for the analysis of G. ussuriensis genetic diversity based on these characteristics. These microsatellites were developed using six multiplex panels, which could be employed to validate 80 G. ussuriensis specimens from different geographical regions in South Korea. The average number of alleles per locus was 12.2 and ranged from 4 to 25 alleles; the observed heterozygosity ranged from 0.238 to 0.950 and the expected heterozygosity ranged from 0.213 to 0.933. As a result of assessing four inland populations, a high level of genetic diversity was confirmed. These newly developed markers will be useful for further studies on the population structure and evolutionary history of the G. ussuriensis.

Keywords

Acknowledgement

Genetic samples for this study were collected under legal permits obtained from the Ministry of Environment, Republic of Korea. Sample collection was approved by the institutional animal care and use committee (approval number: NIBR IACUC 20220001). This work was supported by grants from the National Institute of Biological Resources(NIBR202104102).

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