Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Functional characterization of Clonorchis sinensis choline transporter

  • Jeong Yeon Won (Department of Parasitology and Tropical Medicine, Inha University School of Medicine) ;
  • Johnsy Mary Louis (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Eui Sun Roh (Department of Parasitology and Tropical Medicine, Inha University School of Medicine) ;
  • Seok Ho Cha (Department of Parasitology and Tropical Medicine, Inha University School of Medicine) ;
  • Jin-Hee Han (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University)
  • Received : 2023.08.04
  • Accepted : 2023.09.27
  • Published : 2023.11.30
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Abstract

Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis-Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 µM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF-2017R1D1A1B03034588). Adult Xemopus laevies was obtained from the Korean Xenopus Resource Center for Research.

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