해양환경안전학회지 (Journal of the Korean Society of Marine Environment & Safety)

해양환경안전학회 (The Korean Society of Marine Environment and safety)
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미세조류와 로티퍼를 이용한 멀티모달 생태독성 분석시스템

Multi-modal Eco-toxicity Analyzer Using Microalgae and Rotifer

  • 김형식 (고려대학교 전자.정보공학과 ) ;
  • 신상훈 (고려대학교 전자.정보공학과 ) ;
  • 최훈 (선박해양플랜트연구소 ) ;
  • 강원수 (선박해양플랜트연구소 ) ;
  • 이문진 (선박해양플랜트연구소 ) ;
  • 서성규 (고려대학교 전자.정보공학과)
  • Hyungsik Kim (Department of Electronics and Information Engineering, Korea University) ;
  • Sanghoon Shin (Department of Electronics and Information Engineering, Korea University) ;
  • Hoon Choi (Korea Research Institute of Ships & Ocean Engineering) ;
  • Wonsoo Kang (Korea Research Institute of Ships & Ocean Engineering) ;
  • Moonjin Lee (Korea Research Institute of Ships & Ocean Engineering) ;
  • Sungkyu Seo (Department of Electronics and Information Engineering, Korea University)
  • 투고 : 2023.09.20
  • 심사 : 2023.10.27
  • 발행 : 2023.10.31
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초록

산업과 기술의 발전으로 인해 수계로의 화학물질 배출이 증가하고, 이로 인해 환경오염과 인체 건강에 부정적인 영향을 미치는 위험이 더욱 증가하였다. 따라서, 수질을 종합적으로 평가할 수 있는 생태독성평가의 중요성이 강조되고 있다. 본 연구에서는 렌즈프리 그림자 이미징 기술을 활용한 Cellytics 플랫폼을 소개하며, 화학물질에 의한 로티퍼(Brachionus plicatilis)와 미세조류(Dunaliella tertiolecta)의 생물학적 변화를 신속하게 측정하고 독성을 분석하는 기법을 제안한다. 이를 위해 로티퍼와 미세조류를 톨루엔에 각각 1분과 5분 동안 노출한 뒤, Cellytics를 이용하여 로티퍼의 이동성과 미세조류의 형태 변화를 측정하여 독성을 평가하였다. 로티퍼의 이동성과 미세조류의 형태변화는 모두 110.4 mg/L의 농도에서 대조군과 유의미한 차이를 나타내며(p<0.05), 이는 로티퍼의 생존율로 분석한 톨루엔의 LC50(552 mg/L)보다 낮은 농도였다. 본 연구에 따르면, 로티퍼와 미세조류를 전통적인 방식으로 최소 수 일 간 배양하여 얻을 수 있는 생태독성평가 결과를 매우 짧은 시간(5분 이내)에 분석하고, 두 생물의 독성평가 결과를 신속하게 제공하여 현장에서 활용 가능한 신뢰성 높은 정보를 제공할 수 있음을 보여준다. 이는 독성평가를 이용하는 다양한 연구의 활용에 기여할 수 있으며, 환경보호 및 인체 건강 관련 정책 수립에 도움이 될 것으로 기대된다.

The advancement of industry and technology has led to an increase in the discharge of chemical substances into aquatic ecosystems, resulting in an amplified risk of environmental pollution and negative impacts on human health. Consequently, the importance of comprehensive ecological toxicity assessments for evaluating water quality is being emphasized. This study introduces the Cellytics platform, that uses lens-free shadow imaging technology, and proposes a technique for rapidly measuring and analyzing the biological changes and toxicity induced by chemicals in rotifer (Brachionus plicatilis) and microalgae (Dunaliella tertiolecta). To achieve this, rotifer and microalgae were exposed to toluene for 1 and 5 min, respectively, and their mobility and morphological changes were measured using Cellytics to assess toxicity. The mobility of rotifer and the morphological changes in microalgae showed significant differences compared with those of the control groups at a concentration 110.4 mg/L (p<0.05). These concentrations were lower than the toluene LC50 (552 mg/L) determined through rotifer survival analysis. According to this study, the results of ecological toxicity assessments for rotifer and microalgae could be analyzed in a considerably short time (within 5 min) compared to the traditional approach of culturing them for at least several days. This provides rapidly accessible and highly reliable information that could be used in the field. This contribution is expected to benefit various research applications relying on toxicity assessments and assist in the formulation of policies related to environmental protection and human health.

키워드

과제정보

이 논문은 2023년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구이다(RS-2021-KS211535, 해양 위험유해물질(HNS) 배출 등 관리기술 개발사업, 해양산업시설 배출 위험유해물질 영향평가 및 관리기술 개발).

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