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Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli

  • Xue Yan (School of Life Sciences, Zhejiang Chinese Medical University) ;
  • Liang Jin (School of Life Sciences, Zhejiang Chinese Medical University) ;
  • Huifen Zhou (School of Basic Medicine, Zhejiang Chinese Medical University) ;
  • Haofang Wan (School of Basic Medicine, Zhejiang Chinese Medical University) ;
  • Haitong Wan (School of Life Sciences, Zhejiang Chinese Medical University) ;
  • Jiehong Yang (School of Basic Medicine, Zhejiang Chinese Medical University)
  • Received : 2023.06.13
  • Accepted : 2023.07.17
  • Published : 2023.10.28

Abstract

Infectious diseases caused by drug-resistant Escherichia coli (E. coli) pose a critical concern for medical institutions as they can lead to high morbidity and mortality rates. In this study, amygdalin exhibited anti-inflammatory and antioxidant activities, as well as other potentials. However, whether it could influence the drug-resistant E. coli-infected cells remained unanswered. Amygdalin was therefore tested in a cellular model in which human macrophages were exposed to resistant E. coli. Apoptosis was measured by flow cytometry and the lactate dehydrogenase (LDH) assay. Western immunoblotting and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) were used to quantify interleukin-18 (IL-18), interleukin-1β (IL-1β), and interleukin-6 (IL-6). The production of reactive oxygen species (ROS) in macrophages was detected by ROS kit. The expression of pan-apoptotic proteins in macrophages was measured by qRT-PCR and Western immunoblotting. Drug-Resistant E. coli inhibited cell viability and enhanced apoptosis in the cellular model. In cells treated with amygdalin, this compound can inhibit cell apoptosis and reduce the expression of pro - inflammatory cytokines such as IL-1β, IL-18 and IL-6. Additionally, it decreases the production of PANoptosis proteins, Furthermore, amygdalin lowered the levels of reactive oxygen species induced by drug-resistant E. coli, in cells, demonstrating its antioxidant effects. Amygdalin, a drug with a protective role, alleviated cell damage caused by drug-resistant E. coli in human macrophages by inhibiting the PANoptosis signaling pathway.

Keywords

Acknowledgement

This project was supported by the by the National Natural Science Foundation of China (Grant No. 81930111), and the research was also supported by the Biosafety Laboratory of Integrated Chinese and Western Medicine at Zhejiang Chinese Medicine University (BSL20205713156), the Zhejiang Province Traditional Chinese Medicine Science and Technology project (2023ZF157), and the Research Project of Zhejiang Chinese Medical University (2021RCZXZK23, 2022GJYY025).

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