Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Stimulatory Anticancer Effect of Resveratrol Mediated by G Protein-Coupled Estrogen Receptor in Colorectal Cancer

  • Nayun Kim (College of Pharmacy, Duksung Women's University) ;
  • Junhye Kwon (Department of Radiological & Clinical Research, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences (KIRAMS)) ;
  • Ui Sup Shin (Department of Surgery, Korea Cancer Center Hospital, KIRAMS) ;
  • Joohee Jung (College of Pharmacy, Duksung Women's University)
  • Received : 2023.03.30
  • Accepted : 2023.05.23
  • Published : 2023.11.01
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Abstract

Colorectal cancer (CRC) is one of the most high-risk cancers; however, it has been suggested that estrogen signaling in CRC could have a protective effect. Therefore, we focused on the function of the G protein-coupled estrogen receptor (GPER) among the estrogen receptors in CRC. In this study, we investigated the therapeutic effect of resveratrol via GPER in CRC (RKO and WiDr) cells, CRC cell-derived xenograft models, and organoids (30T and 33T). Resveratrol significantly suppressed cell viability and proliferation in highly GPER-expressing RKO cells compared to that in low GPER-expressing WiDr cells. In xenograft models, resveratrol also delayed tumor growth and exhibited a high survival rate depending on GPER expression in RKO-derived tumors. Furthermore, resveratrol significantly inhibited the viability of organoids with high GPER expression. Additionally, the anticancer effect of resveratrol on CRC showed that resveratrol rapidly responded to GPER, while increasing the expression of p-ERK and Bax and cleaving PARP proteins.

Keywords

Acknowledgement

This research was supported by an NRF grant funded by MSIT (NRF-2021R1A2C2004535), the Priority Research Centers Program through NRF funded by the Ministry of Education (2016R1A6A1A03007648) (J.J.), and a grant of the KIRAMS funded by MSIT, the Republic of Korea (No. 50542-2022) (U.S.).

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