Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Identification of heat shock protein70-2 and protamine-1 mRNA, proteins, and analyses of their association with fertility using frozen-thawed sperm in Madura bulls

  • Zulfi Nur Amrina Rosyada (Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University) ;
  • Berlin Pandapotan Pardede (Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University) ;
  • Ekayanti Mulyawati Kaiin (Research Center for Applied Zoology, National Research and Innovation Agency (BRIN)) ;
  • Ligaya I.T.A Tumbelaka (Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University) ;
  • Dedy Duryadi Solihin (Department of Biology, Faculty of Science, IPB University) ;
  • Bambang Purwantara (Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University) ;
  • Mokhamad Fakhrul Ulum (Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University)
  • Received : 2023.04.14
  • Accepted : 2023.06.14
  • Published : 2023.12.01
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Abstract

Objective: This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers. Methods: The Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate. Results: Relative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality. Conclusion: HSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.

Keywords

Acknowledgement

The authors would like to thank Singosari AI Center, especially Kresno Suharto, Wiwied Sawitri, Anny Amalia, and M.T. Herbowo, and the Lembang AI Center, especially Tri Harsi, Iman, Yessy, and Ida.

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