Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signal-related kinase pathway

  • Nareenath Muneerungsee (Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University) ;
  • Supita Tanasawet (Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University) ;
  • Wanida Sukketsiri (Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University)
  • Received : 2023.03.23
  • Accepted : 2023.06.15
  • Published : 2023.10.01
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Abstract

BACKGROUND/OBJECTIVES: Fucoidan, a polysaccharide content in brown algae, has been reported to inhibit the growth of cancer cells. The present study aimed to investigate the suppression effects of fucoidan on A549 non-small cell lung cancer cells migration. MATERIALS/METHODS: The anti-migratory activity of fucoidan in A549 cells was examined by wound healing assay and phalloidin-rhodamine staining in response to fucoidan (0-100 ㎍/mL) treatment for 48 h. Western blot analysis was performed to clarify the protein expressions relevant to migratory activity. RESULTS: Fucoidan (25-100 ㎍/mL) significantly suppressed A549 cells migration together with reduced the intensity of phalloidin-rhodamine which detect filopodia and lamellipodia protrusions at 48 h of treatment. The protein expression indicated that fucoidan significantly suppressed the phosphorylation of focal adhesion kinase (FAK), Src, and extracellular signal-related kinase (ERK). In addition, the phosphorylation of p38 in A549 cells was found to be increased. CONCLUSIONS: Our data conclude that fucoidan exhibits anti-migratory activities against lung cancer A549 cells mediated by inhibiting ERK1/2 and FAK-Src pathway.

Keywords

Acknowledgement

We would like to acknowledge Faculty of Science, Prince of Songkhla University, Thailand, providing the experimental supports. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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