Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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LED Light Quality Protects Iron Deficiency and Improves Photosynthesis and Biomass Yield in Alfalfa (Medicago sativa L.)

  • Ki-Won Lee (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Sang-Hoon Lee (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Yowook Song (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Yowook Song (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Jae Hoon Woo (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Bo Ram Choi (Grassland and Forage Division, National Institute of Animal Science, RDA) ;
  • Md Atikur Rahman (Grassland and Forage Division, National Institute of Animal Science, RDA)
  • Received : 2023.09.18
  • Accepted : 2023.09.26
  • Published : 2023.09.30
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Abstract

Iron (Fe) is a vital element for plants and other organisms, involving in several physiological processes including respiration, chlorophyll biosynthesis, and photosynthesis. Unfortunately, how Fe accumulation regulates in response to light quality has not been well established in plants. Therefore, the aim of the study was to explore the mechanism of Fe homeostasis by light quality. In this study, we found morpho-physiological attributes were significantly improved in response to blue (λmax: 450) compared to white (λmax: 500) and red (λmax: 660) light. The root-shoot length, plant biomass, photosynthesis efficiency (Fv/Fm) and leafgreen (SPAD) significantly declined in response to white and red light. However, these parameters were improved and iron deficiency was substantially alleviated by blue light exposure in alfalfa seedlings. This study might be useful to the forage breeders and farmers for improving alfalfa yield and nutritional benefits.

Keywords

Acknowledgement

This study was supported by the RDA Fellowship Program of National Institute of Animal Science, and Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01592501), Rural Development Administration, Republic of Korea.

References

  1. Chauhan, J., Srivastava, J.P., Singhal, R.K., Soufan, W., Dadarwal, B.K., Mishra, U.N., Anuragi, H., Rahman, M.A., Sakran, M.I., Brestic, M., Zivcak, M., Skalicky, M. and Sabagh, A.E. 2022. Alterations of oxidative stress indicators, antioxidant enzymes, soluble sugars, and amino acids in mustard [Brassica juncea (L.) Czern and Coss.] in response to varying sowing time, and field temperature. Frontiers in Plant Science. 13.
  2. Das, U., Rahman, M.A., Ela, E.J., Lee, K.W. and Kabir, A.H. 2021. Sulfur triggers glutathione and phytochelatin accumulation causing excess Cd bound to the cell wall of roots in alleviating Cd-toxicity in alfalfa. Chemosphere. 262:128361.
  3. El-Shehawi, A.M., Rahman, M.A., Elseehy, M.M. and Kabir, A.H. 2022. Mercury toxicity causes iron and sulfur deficiencies along with oxidative injuries in alfalfa (Medicago sativa). Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology. 156:284-291. https://doi.org/10.1080/11263504.2021.1985005
  4. Haque, A.F.M.M., Rahman, M.A., Das, U., Rahman, M.M., Elseehy, M.M., El-Shehawi, A.M., Parvez, M.S. and Kabir, A.H. 2022. Changes in physiological responses and MTP (metal tolerance protein) transcripts in soybean (Glycine max) exposed to differential iron availability. Plant Physiology and Biochemistry. 179:1-9. https://doi.org/10.1016/j.plaphy.2022.03.007
  5. Hoagland, D.R. and Arnon, D.I. 1950. The water-culture method for growing plants without soil. Circular California Agricultural Experiment Station. 347:32.
  6. Islam, M.R., Kamal, M.M., Hossain, M.F., Hossain, J., Azam, M.G., Akhter, M.M., Hasan, M.K., Al-Ashkar, I., Almutairi, K.F., Sabagh, A.E., Rahman, M.A., Iqbal, M.A. and Islam, M.S. 2023. Drought tolerance in mung bean is associated with the genotypic divergence, regulation of proline, photosynthetic pigment and water relation. Phyton. 92:955-981. https://doi.org/10.32604/phyton.2023.025138
  7. Kabir, A.H., Ela, E.J., Bagchi, R., Rahman, M.A., Peiter, E. and Lee, K.W. 2023. Nitric oxide acts as an inducer of Strategy-I responses to increase Fe availability and mobilization in Fe-starved broccoli (Brassica oleracea var. oleracea). Plant Physiology and Biochemistry. 194:182-192. https://doi.org/10.1016/j.plaphy.2022.11.018
  8. Kabir, A.H., Rahman, M.A., Rahman, M.M., Brailey-Jones, P., Lee, K.W. and Bennetzen, J.L. 2022. Mechanistic assessment of tolerance to iron deficiency mediated by Trichoderma harzianum in soybean roots. Journal of Applied Microbiology. 133:2760-2778. https://doi.org/10.1111/jam.15651
  9. Kumar, S., Kumar, S. and Mohapatra, T. 2021. Interaction between macro- and micro-nutrients in plants. Frontiers in Plant Science. 12:665583.
  10. Livak, K.J. and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 25:402-408. https://doi.org/10.1006/meth.2001.1262
  11. Rahman, M.A., Ahmed, M.B., Alotaibi, F., Alotaibi, K.D., Ziadi, N., Lee, K.W. and Kabir, A.H. 2021. Growth and physiological impairments in Fe-starved alfalfa are associated with the downregulation of Fe and S transporters along with redox imbalance. Chemical and Biological Technologies in Agriculture. 8:36.
  12. Rahman, M.A., Alam, I., Kim, Y.G., Ahn, N.Y., Heo, S.H., Lee, D.G., Liu, G. and Lee, B.H. 2015. Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach. Plant Physiology and Biochemistry. 89:112-122. https://doi.org/10.1016/j.plaphy.2015.02.015
  13. Rahman, M.A., Bagchi, R., El-Shehawi, A.M., Elseehy, M.M., Anee, S.A., Lee, K.W. and Kabir, A.H. 2022. Physiological and molecular characterization of strategy-I responses and expression of Fe-transporters in Fe-deficient soybean. South African Journal of Botany. 147:942-950. https://doi.org/10.1016/j.sajb.2022.03.052
  14. Rahman, M.A., Kabir, A.H., Mandal, A., Roy, S.K., Song, Y., Ji, H.C. and Lee, K.W. 2020. Glutathione restores Hg-induced morpho-physiological retardations by inducing phytochelatin and oxidative defense in alfalfa. Biology. 9.
  15. Rahman, M.A., Kabir, A.H., Song, Y., Lee, S.H., Hasanuzzaman, M. and Lee, K.W. 2021. Nitric oxide prevents Fe deficiency-induced photosynthetic disturbance and oxidative stress in alfalfa by regulating Fe acquisition and antioxidant defense. Antioxidants. 10:1556.
  16. Rahman, M.A., Kim, Y.G., Alam, I., Gong-she, L., Lee, H., Lee Jeung, J. and Lee, B.H. 2016. Proteome analysis of alfalfa roots in response to water deficit stress. Journal of Integrative Agriculture. 15:1275-1285. https://doi.org/10.1016/S2095-3119(15)61255-2
  17. Rahman, M.A., Parvin, M., Das, U., Ela, E.J., Lee, S.H., Lee, K.W. and Kabir, A.H. 2020. Arbuscular mycorrhizal symbiosis mitigates iron (Fe)-deficiency retardation in alfalfa (Medicago sativa L.) through the enhancement of Fe accumulation and sulfur-assisted antioxidant defense. International Journal of Molecular Sciences. 21:2219.
  18. Rahman, M.A., Woo, J.H., Lee, S.H., Park, H.S., Kabir, A.H., Raza, A., El Sabagh, A. and Lee, K.W. 2022. Regulation of Na+/H+ exchangers, Na+/K+ transporters, and lignin biosynthesis genes, along with lignin accumulation, sodium extrusion, and antioxidant defense, confers salt tolerance in alfalfa. Frontiers in Plant Science. 13:1041764.
  19. Rahman, M.A., Woo, J.H., Song, Y., Lee, S.H., Hasan, M.M., Azad, M.A.K. and Lee, K.W. 2022. Heat shock proteins and antioxidant genes involved in heat combined with drought stress responses in perennial rye grass. Life. 12:1426.