Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Comparative Study of the Neuroprotective Effect of Sihogyeji-tang, Sihosogan-tang, and Sihocheonggan-tang on an MPTP-Induced Parkinson's Disease Mouse Model

MPTP로 유도된 파킨슨병 생쥐 모델에 대한 시호계지탕, 시호소간탕, 시호청간탕의 신경세포 보호 효과 비교 연구

  • Ji Eun Seo (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Hanul Lee (Korean Medicine Research Center for Healthy Aging, Pusan National University) ;
  • Chang-Hwan Bae (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Dong Hak Yoon (KyungHee YDH Oriental Clinic) ;
  • Hee-Young Kim (Korean Medicine Research Center for Healthy Aging, Pusan National University) ;
  • Seungtae Kim (Department of Korean Medical Science, School of Korean Medicine, Pusan National University)
  • 서지은 (부산대학교 한의학전문대학원 한의과학과) ;
  • 이한울 (부산대학교 건강노화한의과학연구센터) ;
  • 배창환 (부산대학교 한의학전문대학원 한의과학과) ;
  • 윤동학 (경희윤동학한의원) ;
  • 김희영 (부산대학교 건강노화한의과학연구센터) ;
  • 김승태 (부산대학교 한의학전문대학원 한의과학과)
  • Received : 2023.06.29
  • Accepted : 2023.08.12
  • Published : 2023.09.27
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Abstract

Objectives : Parkinson's disease (PD) is the second most common neurodegenerative disorder worldwide and is characterized by the loss of the dopaminergic neurons in the substantia nigra (SN). In a previous in vitro study, we demonstrated that Sihogyeji-tang (SG), Sihosogan-tang (SS), and Sihocheonggan-tang (SC) have the potential to be candidate medicines for PD. This study aimed to compare the neuroprotective effect of SG, SS, and SC using 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP)-induced PD mouse model. Methods : Eight-week-old male C57BL/6 mice were intraperitoneally administered with 30 mg/kg of MPTP for 5 days and orally administered SG, SS and SC for 12 days from the first MPTP injection. Motor function was assessed using the pole test and the rotarod test. Dopaminergic neuronal survival in the SN and striatum was evaluated through tyrosine-hydroxylase immunohistochemistry. Results : MPTP administration resulted in behavioral impairment and dopaminergic neuronal death in the SN and striatum. In the pole test, treatment with SG, SS, and SC alleviated the MPTP-induced motor dysfunction on day 5 and 12. In the rotarod test, SS and SG alleviated the MPTP-induced motor dysfunction on day 5, while only SS showed improvement on day 12. SS and SG significantly protected dopaminergic neurons in the SN from MPTP toxicity, and all three compounds (SG, SS, and SC) showed significant protection in the striatum. Notably, SS demonstrated superior efficacy in suppressing MPTP-induced motor dysfunction and dopaminergic neuronal death compared to SG and SC. Conclusions : These findings suggest that SS is the most effective formula among SG, SS, and SC for PD, indicating its potential role in the treatment of PD.

Keywords

Acknowledgement

This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Instiute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number : HF21C0053).

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