DOI QR코드

DOI QR Code

Multivoltine and Bivoltine Silkworm F1 Hybrids Adaptable to Type One (1) Climatic Conditions in the Philippines

  • Marlyn M. Viduya (Don Mariano Marcos Memorial State University) ;
  • Maricris E. Ulat (Don Mariano Marcos Memorial State University) ;
  • Gemma E. Supsup (Don Mariano Marcos Memorial State University) ;
  • Julieta P. Abuan (Don Mariano Marcos Memorial State University) ;
  • Edgar P. Sanchez (Don Mariano Marcos Memorial State University) ;
  • Roel D. Supsup (Don Mariano Marcos Memorial State University)
  • 투고 : 2023.03.29
  • 심사 : 2023.09.21
  • 발행 : 2023.09.30

초록

The eighteen (18) F1 hybrid combinations were tested to identify potential combinations adaptable to type 1 climatic conditions in the Philippines. The six (6) bivoltine purelines (DMMMSU 108, DMMMSU 109, DMMMSU 110, DMMMSU 111, DMMMSU 113, and DMMMSU 119); and three (3) multivoltine purelines (DMMMSU 1000, DMMMSU 1007, and DMMMSU 1014), were crossed (multivoltine x bivoltine) in a mating plan. These were arranged in a Completely Randomized Design (CRD), replicated three times, and analyzed using Analysis of Variance (ANOVA). A test of significance was done using ANOVA across years and Tukey's Honest Significant Difference Test (HSD). The multiple trait evaluation index (EI) method was also used in the identification of potential F1 hybrids. Three major phases were done: (1) parental rearing of multivoltine and bivoltine pure lines for breed multiplication; (2) hybridization process; and (3) evaluation of F1 hybrids. Rearing evaluations were conducted for three consecutive years. Based from the three evaluations, 10 potential crosses were identified: DMMMSU MV-12, DMMMSU MV-11, DMMMSU MV-13, DMMMSU MV-16, DMMMSU MV-07, DMMMSU MV-14, DMMMSU MV-05, DMMMSU MV-09, DMMMSU MV-03, and DMMSU MV-10. The topmost combinations with the best economic and commercial characters and are consistently adaptable during two (2) cropping seasons were DMMMSU MV-07, DMMMSU MV-12, DMMMSU MV-05, DMMMSU MV-09 and DMMMSU MV-11. These newly-identified F1 hybrids are considered potential breeds that could improve cocoon production.

키워드

과제정보

The Authors are ever grateful to Ms. Evangel M. Barrameda, Officer-In-Charge, Office of the Chief of Research and Development Division, for her valuable insights, suggestions, and assistance in the conduct and completion of this endeavor. Likewise, credit is due to the research assistant of the Silkworm Breeding and Improvement Unit. Utmost recognition is addressed to the Don Mariano Marcos Memorial State University for the financial support extended to this humble work.

참고문헌

  1. Benchamin KV, Jolly MS (1986) Principles of silkworm rearing. In Proceedings of the Seminar on Problems and Prospects of Sericulture. Vellore, India (pp. 63-108). 
  2. Cifuentes C, Sohn K (1998) Manual tecnico de la sericultura, cultivo de la morera y cria del gusano de seda en el tropico. Fondo Editorial de Risaralda, Risaralda, Colombia. 
  3. ESCAP (1993) Principles and Techniques of Silkworm Breeding, Bangkok, United Nations Economic and Social Commission for Asia and the Pacific (ST. ESCAP, 1312). 
  4. FAO (2003) Conservation status of sericultural germplasm resources in the World. I. Conservation status of mulberry (Morus spp.) genetic resources in the World. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy. 
  5. Gaviria DA, Aguilar E, Serrano HJ, Alegria AH (2006) DNA fingerprinting using AFLP markers to search for markers associated with yield attributes in the silkworm, Bombyx mori. J Insect Sci 2006, 15. 
  6. Hsieh FK, Yu S, Su SY, Peng SJ (1995) "Studies on the thermo tolerance of the silkworm, Bombyx mori L." Zsongriva.
  7. Kumar KK, Naik SS (2011) Development of polyvoltine× bivoltine hybrids of mulberry silkworm, Bombyx mori L. Tolerant to BmNPV. Int J Zool Res 7, 300. 
  8. Lu C, Dai FY, Xiang ZH (2001) Studies on the mutant strains of the Bombyx mori gene bank. Agric Sci China 116b, 43-48. 
  9. Malik GN, Massoodi MA, Kamili AS, Sofi AM (2006) Studies on heterosis in some bivoltine silkworm (Bombyx mori L.) crosses. J Sericul 6, 2. 
  10. Mano Y (1993) A new method to select promising silkworm breeds/ combinations. Indian J Sericul 31, 53. 
  11. Mirhosseini S, Bizhannia Ar, Vishkaei S, Seydavi A (2007) Investigation on heterosis, general combining ability and special combining ability for cocoon characters in the five local groups and two breeded pure lines of silkworm Bombyx mori L. Iran J Biol 138-143. 
  12. Mubashar H, Khan SA, Muhammad N (2011) Effect of relative humidity on factors of seed cocoon production in some inbred silkworm (Bombyx mori) lines. Int J Agric Biol 13, 57-60. 
  13. Nacheva J, Junka (1989) "Phenotypic and genotypic characterization of silkworm character during the different seasons of silkworm feeding," Genet Sel Evol 22, 242-247. 
  14. Nagalakshmamma K, Jyothi PN (2010) Studies on commercial exploitation of selected multivoltine races of the silkworm Bombyx mori L. in different seasons of Rayalaseema region (AP) in India. BioScan, 5, 31-34. 
  15. Ramesha C, Seshagiri SV, Basha KI, Rao CGP (2008) Synthesis of superior polyvoltine crossbreeds of silkworm (Bombyx mori L.). National Seminar on Scenario of Seri-biotechnological Research in India, August 28-30, S.V. Mahila University, Tirupati, A.P., India. 
  16. Rao, CGP, Basha KI, Seshagiri SV, Ramesh C, Nagaraju H (2004) Identification of Superior Polyvoltine Hybrids (polyvoltine x bivoltine) of Silkworm, Bombyx mori L. Int J Indust Entomol 8, 43-49. 
  17. Ravindra S, Kalpana GV, Rao PS, Ahsan MM, Datta RK, Rekha M (2000) Studies on combining ability and heterosis in the silkworm, Bombyx mori L. Ind J Sericul 39, 43-48. 
  18. Reddy RM, Suresh R, Srivastava AK, Kar PK, Sinha MK, Prasad BC (2010) Heterosis pattern and commercial prospective of assorted F1 hybrids of Indian tropical tasar silkworm, Antheraea mylitta Drury. J Entomol 7, 160-167.  https://doi.org/10.3923/je.2010.160.167
  19. Ruiz H, Almanza M (2018) Implications of genetic diversity in the improvement of silkworm Bombyx mori L., Chilean J Agricul Res 78, 569-579. https://doi.org/10.4067/s0718-5839201800040056 
  20. Rahmathulla VK (2012). Management of climatic factors for successful silkworm (Bombyx mori L.) crop and higher silk production: a review. Psyche 2012, 121234. 
  21. Roa MRP (2016) Selection in silkworm breeding. Guptha Offset Printing, Vidyaranyapuram, Mysuru. 
  22. Saha AK, Datta T, Das SK, Moorthy SM (2008) Bivoltine rearing during adverse season in West Bengal. Indian Silk 47, 5-7. 
  23. Sajgotra M, Bali RK (2016) Expression of heterosis in bivoltine hybrids of Bombyx mori L. for silk yield contributing traits under sub-tropical conditions. Int J Bio-resour Stress Manag 7, 258-262.  https://doi.org/10.23910/IJBSM/2016.7.2.1490b
  24. Seshagiri SV, Ramesha C, Rao CGP (2009) Genetic manifestation of hybrid vigor in cross breeds of mulberry silkworm, Bombyx mori L. Int J Zool Res 5, 150-160.  https://doi.org/10.3923/ijzr.2009.150.160
  25. Shirota T (1992) Selection of healthy silkworm strain through high temperature rearing of fifth instar larvae. Reports of the Silk Science Research Institute (Japan). 
  26. Singh HR, Unni BG, Neog K, Bhattacharyya M (2011) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) based genetic variation studies in eri silkworm (Samia cynthia ricini Lepidoptera: Saturniidae). Afr J Biotechnol 10, 15684-15690.  https://doi.org/10.5897/AJB11.1004
  27. Singh R, Sudhakara, Rao P, Kalpana GV, Basavaraja HK, Ahsan MM, et al. (1998) Studies on hybrid vigour in different crosses of the silkworm Bombyx mori. Sericologia 38, 155-168. 
  28. Singh T, Bhat MM, Khan MA (2009) Insect adaptations to changing environments-temperature and humidity. Int J Ind Entomol 19, 155-164. 
  29. Tajima Y, Ohnuma A (1995) Preliminary experiments on the breeding procedure for synthesizing a high temperature resistant commercial strain of the silkworm, Bombyx mori. Reports of the Silk Science Research Institute (Japan). 
  30. Ueda S, Kimura R, Suzuki K (1975) Studies on the growth of the silkworm, Bombyx mori L., 4: Mutural relationships between the growth in the fifth instar larvae and the productivity of silk substance. Bulletin of Sericultural Experiment Station. 
  31. Ueda S, Lizuka H (1962) Studies on the effects of rearing temperature affecting the health of silkworm larvae and upon the quality of cocoons-1 Effect of temperature in each instar. Acta Sericol Jap 41, 6-21. 
  32. Umadevi K, Sudha VN, Prakashmurthy DP, Raghavendra RD, Ravindra S, Premalatha V, et al. (2005) Evaluation of new multivoltine x bivoltine hybrid "Cauvery"(BL67 x CSR 101) of the silkworm, Bombyx mori L. Indian J Sericul 44, 131-133. 
  33. Venturia N (2002) The effect of environmental conditions on the growth of larvae of silkworm Lucrai., Stiinifice Medicinia Veterinara. Uiversttaea de Stiinte Agricole si Mdeicinia, 45, 544-546. 
  34. Willmer CW, Stone G, Johnston I (2004) Environmental Physiology of Animals, Blackwell Science 2nd edition, Oxford, U.K. 
  35. Zhao Y, Chen K, He S (2007) Key principles for breeding spring-and-autumn using silkworm varieties: from our experience of breeding 873x874. Caspian J Env Sci 5, 57-61.