대한융합한의학회지 (Journal of Convergence Korean Medicine)

대한융합한의학회 (Academy of Convergence Korean Medicine)
권호 표지

고지방식이를 급여한 마우스의 간과 HepG2 세포에서 TJGB의 효과에 대한 연구

Effect of TJGB on the liver of high-fat diet-fed mice and the viability of HepG2 cells

  • 김희영 (경희대학교 약학대학 천연물융합신약개발학과) ;
  • 박예진 (상지대학교 한의과대학 한방재활의학과) ;
  • 안효진 (경희대학교 약학대학 천연물융합신약개발학과)
  • Hee-Young Kim (Department of Integrated Drug Development and Natural Products, Graduate School, Kyung Hee University) ;
  • Yea-Jin Park (Department of Rehabilitative Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sangji University) ;
  • Hyo-Jin An (Department of Integrated Drug Development and Natural Products, Graduate School, Kyung Hee University)
  • 투고 : 2023.06.07
  • 심사 : 2023.06.29
  • 발행 : 2023.06.30
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초록

Objectives: This study was performed to investigate the effect of TJGB on the liver of high-fat diet (HFD)-fed mice and the cell viability of HepG2 cells. Methods: After a week adaptation, 8-week-old C57BL/6N mice were fed with a 45% HFD or normal diet for 3 weeks. For the next 9 weeks, the mice were divided into 6 groups: normal diet group; HFD group; HFD plus orlistat group; HFD plus Ephedra sinica Stapf (ES) group; HFD plus low dose of TJGB group; HFD plus high dose of TJGB group. To estimate the effect of TJGB in the liver of HFD-fed mice, the protein expressions of phospho-acetyl-CoA carboxylase (p-ACC) and liver X Receptor (LXR) were determined by Western blot assay. The cell viability of ES and TJG was also evaluated in HepG2 cells. Results: The administration of TJGB had little effect on the protein expressions of p-ACC and LXR in the liver of HFD-fed mice. And the cytotoxicity was showed above 7.8 ㎍/mL in HepG2 cells. Conclusion: Further research is needed to evaluate the mechanism of TJGB on hepatic steatosis and cytotoxicity in HepG2 cells.

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참고문헌

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